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Opinion of the Scientific Panel on Additives and Products or Substances used in Animal Feed on the modification of terms of authorisation of the micro-organism product Bacillus licheniformis (DSM 5749) and Bacillus subtilis (DSM 5750) (BioPlus 2B) authorised as a feed additive in accordance with Council Directive 70/524/EEC

The European Food Safety Authority (EFSA) received a request to assess the impact of the mixing of the micro-organism product BioPlus 2B with the growth promoter potassium diformate (Formi LHS) in pig feed or the coccidiostat lasalocid A sodium (Avatec 15%) in turkey feed. An in vitro study aimed at determining the susceptibility to potassium diformate of the bacilli vegetative cells was performed using a viable plate count technique. The results showed similar bacilli counts in media with or without potassium diformate 0.5%. However the buffering capacity of the medium used to perform the study may have masked the expected acidic environment. An in vivo growth study involving four experimental groups of 250 one-day old turkeys (control, BioPlus 2B, coccidiostat, BioPlus 2B + coccidiostat) was performed in order to determine the compatibility between the bacterial strains (Bacillus licheniformis, Bacillus subtilis) and the coccidiostat. Despite the numerous zootechnical, biochemical or microbiological parameters studied, the experiment did not make it possible to demonstrate the survival of the bacilli in presence of lasalocid A sodium. In conclusion, the compatibility of BioPlus 2B with either potassium diformate or lasalocid A sodium was not established.

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