Article All rights reserved
refereed
published

Consistent associations with beneficial bacteria in the seed endosphere of barley (Hordeum vulgare L.)

Affiliation
Institute of Applied Microbiology, Research Center for BioSystems, Land Use, and Nutrition (IFZ), Justus-Liebig-University Giessen, Heinrich-Buff-Ring26-32, D-35392 Giessen, Germany
Rahman, M. D. Mahafizur;
Affiliation
Institute of Applied Microbiology, Research Center for BioSystems, Land Use, and Nutrition (IFZ), Justus-Liebig-University Giessen, Heinrich-Buff-Ring26-32, D-35392 Giessen, Germany
Flory, Elias;
Affiliation
Institute of Plant Ecology, Research Center for BioSystems, Land Use, and Nutrition (IFZ), Justus-Liebig-University Giessen, Heinrich-Buff-Ring 26-32,D-35392 Giessen, Germany
Koyro, Hans-Werner;
Affiliation
Institute of Plant Ecology, Research Center for BioSystems, Land Use, and Nutrition (IFZ), Justus-Liebig-University Giessen, Heinrich-Buff-Ring 26-32,D-35392 Giessen, Germany; Institute of Sustainable Halophyte Utilization, University of Karachi, Karachi 75270, Pakistan
Abideen, Zainul;
Affiliation
Julius Kühn-Institut (JKI), Federal Research Centre for Cultivated Plants, Institute for Epidemiology and Pathogen Diagnostics, Braunschweig, Germany
Schikora, Adam;
Affiliation
Institute of Applied Microbiology, Research Center for BioSystems, Land Use, and Nutrition (IFZ), Justus-Liebig-University Giessen, Heinrich-Buff-Ring26-32, D-35392 Giessen, Germany
Suarez, Christian;
Affiliation
Institute of Applied Microbiology, Research Center for BioSystems, Land Use, and Nutrition (IFZ), Justus-Liebig-University Giessen, Heinrich-Buff-Ring26-32, D-35392 Giessen, Germany
Schnell, Sylvia;
Affiliation
Institute of Applied Microbiology, Research Center for BioSystems, Land Use, and Nutrition (IFZ), Justus-Liebig-University Giessen, Heinrich-Buff-Ring26-32, D-35392 Giessen, Germany
Cardinale, Massimiliano

The importance of the plant microbiome for host fitness has led to the concept of the “plant holobiont”. Seeds are reservoirs and vectors for beneficial microbes, which are very intimate partners of higher plants with the potential to connect plant generations. In this study, the endophytic seed microbiota of numerous barley samples, representing different cultivars, geographical sites and harvest years, was investigated. Cultivation-dependent and -independent analyses, microscopy, functional plate assays, greenhouse assays and functional prediction were used, with the aim of assessing the composition, stability and function of the barley seed endophytic bacterial microbiota. Associations were consistently detected in the seed endosphere with Paenibacillus, Pantoea and Pseudomonas spp., which were able to colonize the root with a notable rhizocompetence after seed germination. In greenhouse assays, enrichment with these bacteria promoted barley growth, improved mineral nutrition and induced resistance against the fungal pathogen Blumeria graminis. We demonstrated here that barley, an important crop plant, was consistently associated with beneficial bacteria inside the seeds. The results have relevant implications for plant microbiome ecology and for the holobiont concept, as well as opening up new possibilities for research and application of seed endophytes as bioinoculants in sustainable agriculture.

Files

Cite

Citation style:
Could not load citation form.

Access Statistic

Total:
Downloads:
Abtractviews:
Last 12 Month:
Downloads:
Abtractviews:

Rights

License Holder: 2018 Elsevier GmbH.

Use and reproduction:
All rights reserved