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Biological properties of Beet soil-borne mosaic virus and Beet necrotic yellow vein virus cDNA clones produced by isothermal in vitro recombination: Insights for reassortant appearance

Affiliation
Institute of Sugar Beet Research, Dept. of Phytopathology, 37079 Göttingen, Germany
Laufer, Marlene;
Affiliation
Institute of Horticultural Production Systems, Dept. Phytomedicine, Plant Virology, Leibniz University, 30419 Hannover, Germany
Mohammad, Hamza;
Affiliation
Institute of Horticultural Production Systems, Dept. Phytomedicine, Plant Virology, Leibniz University, 30419 Hannover, Germany
Maiss, Edgar;
GND
172616271
Affiliation
Julius Kühn-Institute (JKI), Federal Research Centre for Cultivated Plants, Institute for Epidemiology and Pathogen Diagnostics, Brunswick, Germany
Richert-Pöggeler, Katja;
Affiliation
DipSA-Plant pathology, University of Bologna, Viale G. Fanin, 40, 40127 Bologna, Italy ; Institut de biologie moléculaire des plantes, CNRS UPR2357, Université de Strasbourg, Strasbourg, France
Dall'Ara, Mattia;
Affiliation
DipSA-Plant pathology, University of Bologna, Viale G. Fanin, 40, 40127 Bologna, Italy
Ratti, Claudio;
Affiliation
Institut de biologie moléculaire des plantes, CNRS UPR2357, Université de Strasbourg, Strasbourg, France
Gilmere, David;
Affiliation
Institute of Sugar Beet Research, Dept. of Phytopathology, 37079 Göttingen, Germany
Liebe, Sebastian;
Affiliation
Institute of Sugar Beet Research, Dept. of Phytopathology, 37079 Göttingen, Germany
Varrelmann, Mark

Two members of the Benyviridae family and genus Benyvirus, Beet soil-borne mosaic virus (BSBMV) and Beet necrotic yellow vein virus (BNYVV), possess identical genome organization, host range and high sequence similarity; they infect Beta vulgaris with variable symptom expression. In the US, mixed infections are described with limited information about viral interactions. Vectors suitable for agroinoculation of all genome components of both viruses were constructed by isothermal in vitro recombination. All 35S promoter-driven cDNA clones allowed production of recombinant viruses competent for Nicotiana benthamiana and Beta macrocarpa systemic infection and Polymyxa betae transmission and were compared to available BNYVV B-type clone. BNYVV and BSBMV RNA1 + 2 reassortants were viable and spread long-distance in N. benthamiana with symptoms dependent on the BNYVV type. Small genomic RNAs were exchangeable and systemically infected B. macrocarpa. These infectious clones represent a powerful tool for the identification of specific molecular host-pathogen determinants.

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License Holder: 2018 Elsevier Inc.

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