Revisiting Francisella tularensis subsp. holarctica, causative agent of tularemia in Germany with bioinformatics: New insights in genome structure, DNA methylation and comparative phylogenetic analysis

Busch, Anne; Thomas, Prasad GND; Zuchantke, Eric GND; Brendebach, Holger; Neubert, K.; Gruetzke, Josephine; Al Dahouk, Sascha; Peters, M.; Hotzel, Helmut GND; Neubauer, Heinrich Karl Johann GND; Tomaso, Herbert GND

Francisella (F.) tularensis is a highly virulent, Gram-negative bacterial pathogen and the causative agent of the zoonotic disease tularemia. Here, we generated, analyzed and characterized a high quality circular genome sequence of the F. tularensis subsp. holarctica strain 12T0050 that caused fatal tularemia in a hare. Besides the genomic structure, we focused on the analysis of oriC, unique to the Francisella genus and the first report on genomic DNA methylation of a Francisella strain. The high quality genome was used to establish and evaluate a diagnostic whole genome sequencing pipeline. A genotyping strategy for F. tularensis was developed using various bioinformatics tools for genotyping. Additionally, whole genome sequences of F. tularensis subsp. holarctica isolates isolated in the years 2008-2015 in Germany were generated. A phylogenetic analysis allowed to determine the genetic relatedness of these isolates and confirmed the highly conserved nature of F. tularensis subsp. holarctica.

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Busch, Anne / Thomas, Prasad / Zuchantke, Eric / et al: Revisiting Francisella tularensis subsp. holarctica, causative agent of tularemia in Germany with bioinformatics: New insights in genome structure, DNA methylation and comparative phylogenetic analysis. 2018.

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