Scientific Opinion on the compatibility of the microbial product 035 ( Bacillus subtilis) with lasalocid sodium, maduramycin ammonium, monensin sodium, narasin, salinomycin sodium and semduramycin sodium

Following a request from the European Commission, the European Food Safety Authority (EFSA) was asked to deliver an opinion on the compatibility of microbial feed additive 035 based on Bacillus subtilis (DSM 17299) with the coccidiostats lasalocid sodium, maduramycin ammonium, monensin sodium, narasin, salinomycin sodium and semduramycin sodium. While the compatibility of Bacillus subtilis (DSM 17299) with lasalocid sodium has been established in a previous assessment of the product BioPlus 2B containing the same strain, the Panel on Additives and Products or Substances used in Animal Feed (FEEDAP) was unable to conclude on the compatibility with the rest of the coccidiostats. The applicant has now provided new data to support the compatibility of the product 035 with the coccidiostats mentioned above. Two studies have been provided, involving 560 and 600 chickens for a period of 34 and 28 days, respectively. The first trial was performed with the product BioPlus 2B, while the second one was done with 035. In both cases, the birds received a basal diet containing B. subtilis DSM 17299 at the recommended inclusion rate in the presence or absence of the coccidiostats monensin sodium, salinomycin sodium and semduramycin sodium (trial 1) or salinomycin sodium, narasin, monensin sodium and maduramycin ammonium (trial 2) at their maximum authorised levels. At the end of the experiments caecal samples were randomly collected and analysed for B. subtilis applying a heat treatment to distinguish between the vegetative cells and spores. No significant differences in the CFU counts were observed between the control and the treated groups in any of the two trials. The FEEDAP Panel concludes that the product 035 (B. subtilis DSM 17299) is compatible with lasalocid sodium, maduramycin ammonium, monensin sodium, narasin, salinomycin sodium and semduramycin sodium.