Article CC BY 4.0
refereed
published

VIM-1 carbapenemase-producing Escherichia coli isolated from retail seafood, Germany 2016.

Affiliation
Freie Universitaet Berlin, Institute for Animal Hygiene and Environmental Health, Berlin, Germany.
Roschanski, Nicole;
Affiliation
Freie Universitaet Berlin, Institute for Animal Hygiene and Environmental Health, Berlin, Germany.
Guenther, Sebastian;
Affiliation
Freie Universitaet Berlin, Institute of Food Safety and Food Hygiene, Berlin, Germany.
Vu, Thi Thu Tra;
Affiliation
Federal Institute for Risk Assessment, Department Biological Safety, Berlin, Germany.
Fischer, Jennie;
Affiliation
Robert Koch Institute, Berlin, Germany.
Semmler, Torsten;
Affiliation
Freie Universitaet Berlin, Institute of Food Safety and Food Hygiene, Berlin, Germany.
Huehn, Stephan;
Affiliation
Freie Universitaet Berlin, Institute of Food Safety and Food Hygiene, Berlin, Germany.
Alter, Thomas;
Affiliation
Freie Universitaet Berlin, Institute for Animal Hygiene and Environmental Health, Berlin, Germany.
Roesler, Uwe

Carbapenems belong to the group of last resort antibiotics in human medicine. Therefore, the emergence of growing numbers of carbapenemase-producing bacteria in food-producing animals or the environment is worrying and an important concern for the public health sector. In the present study, a set of 45 Enterobacteriaceae isolated from German retail seafood (clams and shrimps), sampled in 2016, were investigated by real-time PCR for the presence of carbapenemase-producing bacteria. One Escherichia coli (ST10), isolated from a Venus clam (Ruditapes philippinarum) harvested in the Mediterranean Sea (Italy), contained the carbapenemase gene blaVIM-1 as part of the variable region of a class I integron. Whole-genome sequencing indicated that the integron was embedded in a Tn3-like transposon that also contained the fluoroquinolone resistance gene qnrS1. Additional resistance genes such as the extended-spectrum beta-lactamase blaSHV-12 and the AmpC gene blaACC-1 were also present in this isolate. Except blaACC-1, all resistance genes were located on an IncY plasmid. These results confirm previous observations that carbapenemase-producing bacteria have reached the food chain and are of increasing concern for public health.

Cite

Citation style:
Could not load citation form.

Access Statistic

Total:
Downloads:
Abtractviews:
Last 12 Month:
Downloads:
Abtractviews:

Rights

Use and reproduction:

Export