Vergleichende Serumuntersuchungen mit ELISA-Kits für den Antikörpernachweis gegen das bovine Leukosevirus

Straub, Otto GND; Bruchhof, B. R.

Blood and sera from cattle at various stages of infection with bovine leukosis virus were examined for the presence of antibodies using four different ELISA kits and the immuno-diffusion test after determining their haematological and antigenic status. Uninfected cattle were used as controls. In the majority of cases, particularly seronegative animals, there was agreement between the results. Differences were observed in weakly positive animals and in two animals derived from positive cows. These had an elevated lymphocyte count, a negative reaction in the immuno-diffusion test and different results in the ELISA kits. The polymerase chain reaction should differentiate between possible false positive results and a lack of sensitivity in some of the ELISA kits. It is necessary to carry out comparative trials between the producers of the kits and diagnostic laboratories to obtain standardised results and prevent undue consequences for herds. A change in the eradication programme is also indicated. The number of lymphocytes should be determined in seropositive cattle in herds previously cleared of seropositive animals. Where elevated counts are found the offspring of such animals should be removed

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Straub, Otto / Bruchhof, B.: Vergleichende Serumuntersuchungen mit ELISA-Kits für den Antikörpernachweis gegen das bovine Leukosevirus. 1994.

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