Black poplar (Populus nigra L.) is a keystone species of riparian softwood forests along riversides in vast areas of Europe, Western Asia and Northern Africa. Since the end of the 20th century, black poplar has been recognized as an endangered species throughout Europe due to the loss of its natural habitat and possible crossbreeding with hybrid poplars. Using twelve nuclear SSR loci, we analysed the genetic structure of four native populations from three river valleys in the northern part of Serbia. All tested loci were highly polymorphic, displaying 8 to 25 alleles per locus, overall 179 detected alleles and an zygosity (overall Ho = 0.703) has been lower than the expected
(overall He = 0.808) in each population, which indicates positive Fis > 0 (0.132)). An AMOVA analysis revealed that the highest degree of genetic variation occurred within populations (95.33 %) while the genetic variation between populations was really low (4.67 %). been recorded in the studied populations in Serbia .
