Analysis of the Cellular Stress Response During Ebola Virus Infection by Immunofluorescence

Nelson, E., V.; Schmidt, Kristina, Maria

doi:10.1007/978-1-4939-7116-9_17

Veröffentlicht

Analysis of the Cellular Stress Response During Ebola Virus Infection by Immunofluorescence

In this chapter, the use of immunofluorescence analysis as a tool to examine stress granule (SG) formation in Ebola virus (EBOV)-infected cells is described. The following protocol focuses on the process of inducing and analyzing the cellular stress response, including treatment of cells with inducers and inhibitors of the SG formation, and also describes EBOV infection, DNA transfection, and the usage of different cell lines.

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Einordnung

Erschienen in:: Ebolaviruses : Methods and Protocols
(2017), S. 211-225
Datum der Veröffentlichung:: 02.06.2017
DOI:: 10.1007/978-1-4939-7116-9_17
Sprache:: Englisch
Ressourcentyp:: Text
Schlagwörter:: Zaire ebolavirus; Ebola virus; Filoviruses; Stress granules; Cellular stress response; Immunofluorescence; Microscopy; Antibodies; Sodium arsenite
DDC-Sachgruppe der DNB:: 570 Biowissenschaften, Biologie
Link URL:: https://link.springer.com/protocol/10.1007/978-1-4939-7116-9_17
Einrichtung:: Friedrich-Loeffler-Institut, Institut für neue und neuartige Tierseuchenerreger
Physischer Standort:: SD/2017/240