Detection of Foot-and-Mouth Disease virus in nasal swabs of asymptomatic cattle by RT-PCT within 24 hours

Marquardt, Otfried; Straub, Otto; Ahl, Reinhard; Haas, Bernd

doi:10.1016/0166-0934(95)00015-M

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Detection of Foot-and-Mouth Disease virus in nasal swabs of asymptomatic cattle by RT-PCT within 24 hours

Marquardt, Otfried ; Straub, Otto ; Ahl, Reinhard ; Haas, Bernd

A method for extracting RNA from animal-derived materials that provides foot-and-mouth disease viral template suitable for Tth polymerase-dependent synthesis of cDNA and subsequent PCR is described. Viral genomes were detected in less than 24 h. Nasal swabs that can be easily and repeatedly collected, proved suitable for virus detection by PCR, even during the asymptomatic stages of infection.

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Erschienen in:: Journal of Virological Methods
Vol. 53, H. 2-3S. 255-261
Band:: 53
Heft:: 2-3
Datum der Veröffentlichung:: 1995
DOI:: 10.1016/0166-0934(95)00015-M
Sprache:: Englisch
Ressourcentyp:: Text
Schlagwörter:: Foot-and-mouth disease (FMD); Polymerase chain reaction; Nasal swab; Diagnosis
DDC-Sachgruppe der DNB:: 570 Biowissenschaften, Biologie
Einrichtung:: Friedrich-Loeffler-Institut, Vorgängereinrichtung
Physischer Standort:: SD/1995/66