An air-liquid interphase approach for modeling the early embryo-maternal contact zone

Chen, S.; Palma-Vera, S. E.; Langhammer, M.; Galuska, S. P.; Braun, B. C.; Krause, E.; Lucas-Hahn, Andrea GND; Schoen, J.

We developed an air-liquid interphase culture procedure for mammalian oviduct epithelial cells leading to the formation of functional epithelial tissues, which generate oviduct fluid surrogates. These in vitro oviduct epithelia can be co-cultured with living zygotes and enable embryonic development up to the blastocyst stage without addition of embryo culture medium. The described strategy is broadly applicable to analyze early embryo-maternal interactions under standardized in vitro conditions.

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Chen, S. / Palma-Vera, S. / Langhammer, M. / et al: An air-liquid interphase approach for modeling the early embryo-maternal contact zone. 2017.

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