Early changes in the phenotypic composition of lymphocytes in the bronchoalveolar lavage of pigs after aerogenic immunization with Pasteurella multocida aerosols

Köhler, Heike GND; Lemser, B.; Müller, G.; Saalmüller, Armin GND

The phenotypic composition of bronchoalveolar lymphocytes (BALL) of healthy untreated pigs and of pigs immunized via aerosol with a live Pasteurella multocida mutant was measured cytofluorometrically in comparison to blood lymphocytes (PBL). Cells were analyzed using monoclonal antibodies against porcine IgM, IgA, IgG and SWC3 in single-colour fluorescence and the following antibody combinations for two-colour fluorescence; CD2SWC1, SWC1CD25 and CD4CD8. In nonimmunized pigs, differences between lymphocyte subsets derived from blood and lung were obvious. The numbers of CD2−SWC1+ T-cells, CD25+ activated lymphocytes and IgG+ cells were significantly higher in the bronchoalveolar compartment. Aerosol immunization with P. multocida caused only insignificant changes in the phenotypic composition of PBL whereas the composition of lung-derived lymphocytes changed markedly. The percentage of CD2−SWC1+ lymphocytes and of CD25+ cells coexpressing the SWC1 antigen in BALL increased up to 10 days after aerosol administration, whereas the percentage of all Ig+ cells decreased. These data reflect an early cellular reaction to an aerogenic immunization with a P. multocida aerosol, characterized by an increase in CD2− T-cells and a T-cell activation.

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Köhler, H. / Lemser, B. / Müller, G. / et al: Early changes in the phenotypic composition of lymphocytes in the bronchoalveolar lavage of pigs after aerogenic immunization with Pasteurella multocida aerosols. 1997.

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