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Complete genomic sequence of barley ( Hordeum vulgare) endornavirus (HvEV) determined by next-generation sequencing

Affiliation
NRA, UMR 1332 Biologie du Fruit et Pathologie, CS 20032, 33882 Villenave d’Ornon Cedex, France
Candresse, Thierry;
Affiliation
NRA, UMR 1332 Biologie du Fruit et Pathologie, CS 20032, 33882 Villenave d’Ornon Cedex, France
Marais, Armelle;
Affiliation
Dipartimento di Agraria, Universita` degli Studi di Napoli ‘‘Federico II’’, 80055 Portici, Italy
Sorrentino, Roberto;
Affiliation
NRA, UMR 1332 Biologie du Fruit et Pathologie, CS 20032, 33882 Villenave d’Ornon Cedex, France
Faure, Chantal;
Affiliation
NRA, UMR 1332 Biologie du Fruit et Pathologie, CS 20032, 33882 Villenave d’Ornon Cedex, France
Theil, Sebastien;
Affiliation
GEVES, 25 rue Georges Morel, CS 90024, 49071 Beaucouze´ Cedex, France
Cadot, Valerie;
Affiliation
GEVES, 25 rue Georges Morel, CS 90024, 49071 Beaucouze´ Cedex, France
Rolland, Mathieu;
Affiliation
GEVES, 25 rue Georges Morel, CS 90024, 49071 Beaucouze´ Cedex, France
Villemot, Julie;
GND
1058968432
Affiliation
Institute for Epidemiology and Pathogen Diagnostics, Julius Kuhn-Institute, Federal Research Institute for Cultivated Plants, Erwin-Baur-Straße 27, 06484 Quedlinburg, Germany
Rabenstein, Frank

Endornaviruses are unusual plant-, fungus- and oomycete-infecting viruses with a large, ca 14- to 17-kb linear double-stranded RNA (dsRNA) genome and a persistent lifestyle. The complete genome sequence of an endornavirus from the barley (Hordeum vulgare) Nerz variety was determined from paired Illumina MySeq reads derived from purified dsRNAs. The genome is 14,243 nt long, with 5’ and 3’ non-coding regions of 207 and 47 nt, respectively. It encodes a single large protein of 4663 amino acids that carries conserved motifs for a methyltransferase, a helicase and an RNA-dependent RNA polymerase. The sequence of Hordeum vulgare endornavirus (HvEV) carries all the hallmarks of a typical member of the genus Endornavirus, with the exception of an UDP-glycosyltransferase motif observed in many, but not all, endornaviral genomes.

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