Sequence and initial characterization of the UL10 (glycoprotein M) and UL11 homologous genes of serotype 1 Marek's Disease Virus

Osterrieder, Nikolaus GND

The nucleotide sequence of the U-L 10 (glycoprotein M) and the U-L 11 homologs of Marek's Disease Virus 1 strain GA was determined. The UL10 open reading frame encodes a type III membrane protein of 424 amino acids that contains eight hydrophobic domains and two consensus N-linked glycosylation sites. The U(L)11 homologous gene encodes an 84 amino acid polypeptide, and contains a highly conserved myristylation site at its aminoterminus. By analysis of infected-cell RNA with strand-specific RNA probes, transcription of both U(L)10 and U-L 11 in infected cells was demonstrated. Coupled in vitro transcription-translation confirmed that the U-L 10 product is a 47 kD N-glycosylated viral protein that aggregated upon boiling, whereas the U-L 11 protein exhibited a size of 12 kD after in vitro translation

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Osterrieder, Nikolaus: Sequence and initial characterization of the UL10 (glycoprotein M) and UL11 homologous genes of serotype 1 Marek's Disease Virus. 1999.

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