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Nuclear DNA content variation within the genus Daucus (Apiaceae) determined by flow cytometry

GND
1071425048
Affiliation
Department of Cell Biology, The Franciszek Gorski Institute of Plant Physiology, Polish Academy of Sciences, Niezapominajek 21, PL 30-239 Krakow, Poland
Nowicka, Anna Małgorzata;
Affiliation
Department of Genetics, Physiology and Biotechnology, UTP University of Science and Technology, Prof. S. Kaliskiego Ave. 7, PL 85-789, Bydgoszcz, Poland
Sliwinska, Elwira;
Affiliation
Institute of Plant Biology and Biotechnology, Faculty of Biotechnology and Horticulture, University of Agriculture in Krakow, Al. 29 Listopada 54, PL31-425 Krakow, Poland
Grzebelus, Dariusz;
Affiliation
Institute of Plant Biology and Biotechnology, Faculty of Biotechnology and Horticulture, University of Agriculture in Krakow, Al. 29 Listopada 54, PL31-425 Krakow, Poland
Baranski, Rafal;
Affiliation
United States Department of Agriculture − Agricultural Research Service, Vegetable Crops Research Unit, University of Wisconsin, 1575 Linden Drive, Madison, WI 53706-1590, USA
Simond, Philipp W.;
GND
1059150638
Affiliation
Julius Kühn-Institute (JKI), Institute for Breeding Research on Horticultural Crops, Germany
Nothnagel, Thomas;
Affiliation
Institute of Plant Biology and Biotechnology, Faculty of Biotechnology and Horticulture, University of Agriculture in Krakow, Al. 29 Listopada 54, PL31-425 Krakow, Poland
Grzebelus, Ewa

The genus Daucus (Apiaceae) comprises species from around the world, covering a wide climatic range, and showing great morphological plasticity. Both cultivated and wild forms are described within the genus. The aim of the present study was to estimate the genome size variability in the collection of diploid Daucus species differing in chromosome number (2n = 18, 20 or 22) and originating from various regions of the world. In total, the 2C DNA content in 19 accessions of nine wild Daucus species, as well as in 22 wild and 26 cultivated accessions of Daucus carota L. was measured by flow cytometry. The 2C DNA content varied over 3-fold, from 0.920 pg in D. carota subsp. maximus (2n = 18, Spain) to 3.019 pg in D. littoralis (2n = 20, Israel). The interspecific genome size differences within wild Daucus species were pronounced, e.g. D. carota (2n = 18, Greece) possessed 0.940 pg/2C DNA, D. broteri (2n = 20, Cyprus) 2.218 pg/2C and D. montevidensis (2n = 22, South America) 1.295 pg/2C. In the group of 22-chromosome Daucus species, high similarity in nuclear DNA content among different accessions of one species was observed, in contrast to the 20-chromosome Daucus species, suggesting possible taxonomical misclassifications of accessions within the latter group. The nuclear DNA content of non-cultivated forms of D. carota varied from 0.920 pg/2C in subspecies maximus (Spain) to 1.154 pg/2C in subspecies halophilus (Portugal), which reflects 20% difference. In turn, in the group of cultivated carrots we observed a high homogeneity among the studied accessions, the 2C DNA content ranged between 0.950 pg (‘Kuroda type II’, China; ‘Imperial Long Scarlet’, Japan; ‘Koral’, Poland) and 0.977 pg (‘Cape Market’, South Africa). The mean 2C value calculated for both wild and cultivated forms of D. carota amounted 0.973 pg.

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