Electrochemiluminescence (ECL) immunosensor for detection of Francisella tularensis on screen-printed gold electrode array

Spehar-Délèze, A.-M.; Julich, Sandra GND; Gransee, R.; Tomaso, Herbert GND; Dulay, S.B.; O’Sullivan, C.K.

An electrochemiluminescence (ECL) immunosensor for the rapid detection of the Francisella tularensis pathogen using whole antibodies or antibody fragments as capture biomolecule is described. A sandwich immunoassay was used with either lipopolysaccharide (LPS) or the whole inactivated bacterial cell (LVS) as a target, while Ru(bpy)3 2+-encapsulated silicate nanoparticles were linked to the secondary antibody and used as ECL labels. The assay was performed in a fluidic chip housed in a custom-built black box incorporating electronics, optics and fluidics. The obtained limit of detection for LPS was 0.4 ng/mL, while for the LVS it was 70 and 45 bacteria/mL when the capturing molecule was the whole antibody and the antibody F(ab) fragment, respectively.

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Spehar-Délèze, A.-M. / Julich, Sandra / Gransee, R. / et al: Electrochemiluminescence (ECL) immunosensor for detection of Francisella tularensis on screen-printed gold electrode array. 2016.

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