Die Isolierung und Kultivierung des Q-Fieber-Erregers (Coxiella burnetii) mittels Zellkulturen

Infection with Coxiella burnetii is frequently associated with abortion in ruminants. It is also the aetiological agent of fever in man and is therefore a zoonosis. Coxiella burnetii ran be isolated in cell culture. Microbial contaminants are separated by a filtration step. Propagatian of Coxiella burnetii far antigen preparation is performed using serum-free media. The method allows inactivation of the agent by hearing without the problem of protein precipitation