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Exploring the complex response to linuron of bacterial communities from biopurification systems by means of cultivation-independent methods

GND
1176050842
Affiliation
Julius Kühn-Institute (JKI), Federal Research Centre for Cultivated Plants, Institute for Epidemiology and Pathogen Diagnostics, Brunswick, Germany ; Civil Engineering Department, Catholic University of Rio de Janeiro, Rua Marques de Sao Vicente 225/301-L, Gavea, 22453-900, Rio de Janeiro, RJ, Brazil
Dealtry, Simone;
GND
1119102766
Affiliation
Julius Kühn-Institute (JKI), Federal Research Centre for Cultivated Plants, Institute for Epidemiology and Pathogen Diagnostics, Brunswick, Germany
Nour, Eman H.;
Affiliation
Section for Microbiology, Department of Biology, University of Copenhagen, Copenhagen, Denmark
Holmsgaard, Peter N.;
GND
102059845X
Affiliation
Julius Kühn-Institute (JKI), Federal Research Centre for Cultivated Plants, Institute for Epidemiology and Pathogen Diagnostics, Brunswick, Germany
Ding, Guo-Chun;
GND
1186945141
Affiliation
Julius Kühn-Institute (JKI), Federal Research Centre for Cultivated Plants, Institute for Epidemiology and Pathogen Diagnostics, Brunswick, Germany
Weichelt, Viola;
Affiliation
Division of Soil and Water Management, KU Leuven, Heverlee, Belgium
Dunon, Vincent;
GND
1058940058
Affiliation
Julius Kühn-Institute (JKI), Federal Research Centre for Cultivated Plants, Institute for Epidemiology and Pathogen Diagnostics, Brunswick, Germany
Heuer, Holger;
Affiliation
Section for Microbiology, Department of Biology, University of Copenhagen, Copenhagen, Denmark
Hansen, Lars H.;
Affiliation
Section for Microbiology, Department of Biology, University of Copenhagen, Copenhagen, Denmark
Sørensen, Søren J.;
Affiliation
Division of Soil and Water Management, KU Leuven, Heverlee, Belgium
Springae, Dirk;
GND
1058967878
Affiliation
Julius Kühn-Institute (JKI), Federal Research Centre for Cultivated Plants, Institute for Epidemiology and Pathogen Diagnostics, Brunswick, Germany
Smalla, Kornelia

On-farm biopurification systems (BPSs) treat pesticide-contaminated wastewater at farms through biodegradation and sorption processes. However, information on the microbiota involved in pesticide removal in BPSs is scarce. Here we report on the response of BPS bacterial communities to the herbicide linuron (BPS+) compared with the control (BPS−) in a microcosm experiment. Both denaturing gradient gel electrophoresis (DGGE) and pyrosequencing of 16S rRNA gene fragments amplified from community DNA indicated shifts in the bacterial community after linuron application. Responding populations belonged to taxa that were previously reported from linuron degrading consortia cultivated from soil (Hyphomicrobiaceae, Comamonadaceae, Micrococcaceae). In addition, numerous taxa with increased relative abundance were identified that were previously not associated with linuron degradation. The relative abundance of IncP-1 korB copies increased in response to linuron application. Amplicon pyrosequencing of IncP-1 trfA genes revealed a high IncP-1 plasmid diversity and suggested that populations carrying IncP-1β plasmids increased in relative abundance. Transferable mercury resistance plasmids were exogenously captured from BPS+/BPS−, and in three transconjugants from BPS+ the gene hylA was detected. Our data suggest the existence of a multispecies linuron degrading bacterial food web and an involvement of IncP-1 plasmids in the adaptation of bacterial communities to pesticide pollution in BPSs.

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