Glycoprotein M of bovine herpesvirus 1 (BHV-1) is nonessential for replication in cell culture and is involved in inhibition of bovine respiratory syncytial virus F protein induced syncytium formation in recombinant BHV-1 infected cells

König, Patricia GND; Giesow, Katrin GND; Keil, Günther M. GND

Cell cultures infected with BHV-1/F-syn, a recombinant bovine herpesvirus 1 (BHV-1) which expresses a synthetic open reading frame encoding the fusion (F) protein of the bovine respiratory syncytial virus (BRSV), showed a cytopathic effect (CPE) indistinguishable from that induced by wildtype BHV-1 although transient transfection experiments demonstrated that expression of the F protein leads to formation of large syncytia. Since it has been shown that glycoprotein M (am) of pseudorabies virus inhibits BRSV F-induced syncytium formation in transient plasmid transfection experiments [Pseudorbies virus glycoprotein M inhibits membrane fusion. J. Virol. 74 (2000) 6760], the am ORF of wtBHV-1 and BHV-1/F-syn was interrupted. Infection of cell cultures with the resulting gM(-) mutant of BHV- 1/F-syn, led to formation of syncytia, whereas the CPE in gM(-)BHV-1 infected cells was comparable to the CPE in wtBHV-1 infected cultures. Our results demonstrate that am is not essential for BHV-1 replication in cell culture and that am is involved in inhibition of the cell fusion activity of the BHV-1 expressed BRSV F protein.

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König, Patricia / Giesow, Katrin / Keil, Günther M.: Glycoprotein M of bovine herpesvirus 1 (BHV-1) is nonessential for replication in cell culture and is involved in inhibition of bovine respiratory syncytial virus F protein induced syncytium formation in recombinant BHV-1 infected cells. 2002.

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