In vitro metabolism of the cyanotoxin cylindrospermopsin in HepaRG cells and liver tissue fractions

Kittler, K.; Hurtaud-Pessel, D.; Maul, R.; Kolrep, F.; Fessard, V.

No evidence for phase I metabolites of the cyanotoxin cylindrospermopsin (CYN) was given using HepaRG cells and different liver tissue fractions when studying metabolic conversion. Although the application of ketoconazole, a CYP3A4 inhibitor, led to a decreased cytotoxicity of CYN, no metabolites were detected applying high resolution mass spectrometry. Quantification of non-modified CYN led to recovery rates of almost 100%. Consequently, reduction of CYN toxicity in the presence of metabolism inhibiting agents must be attributed to alternative pathways.

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Kittler, K. / Hurtaud-Pessel, D. / Maul, R. / et al: In vitro metabolism of the cyanotoxin cylindrospermopsin in HepaRG cells and liver tissue fractions. 2016.

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