Different counteracting host immune responses to clade 126.96.36.199 and 188.8.131.52 Egyptian H5N1 highly pathogenic avian influenza viruses in naive and vaccinated chickens
In Egypt, two distinct lineages of H5N1 highly pathogenic avian influenza (HPAI) viruses, “classic 184.108.40.206” and “variant 220.127.116.11” strains, have evolved. The underlying host immune responses counteracting these viruses in chickens remain not well understood. In the present study, the cytokine responses to a classic strain (C121) and those to a variant strain (V1063) were compared in naïve and vaccinated chickens. In naïve chickens, the C121 replicated more efficiently than the V1063. Both the C121 and the V1063 increased interferon (IFN)-gamma and interleukin (IL)-10 gene expression at 48 hours post inoculation (hpi) in the lung and spleen but the levels of these cytokines were lower in chickens infected with the C121 than those infected with the V1063. In contrast, in chickens vaccinated with inactivated C121-based vaccine, the C121 replicated less than the V1063. Both challenge with the C121 and that with the V1063 did not increase IFN-gamma gene expression at 48 hpi; rather, the C121 increased IL-4 gene expression in the lung accompanied with lower viral titer and higher HI titers. These results suggested that the pathogenicity of HPAI viruses correlated with IFN-gamma-producing helper and/or cytotoxic T cell responses in naïve chickens, whereas vaccine efficacy to HPAI viruses correlated with IL-4 producing helper T cell responses in the lung in vaccinated chickens. It implies that IL-4 in the lung, in addition to the traditional serum HI titers, could be used to screen novel vaccine strategies, such as strains, adjuvant, prime/boost protocols, against HPAI in chickens.
Samy, A.A. / El-Enbaawy, M. / El-Sanousi, A.A. / et al: Different counteracting host immune responses to clade 18.104.22.168 and 22.214.171.124 Egyptian H5N1 highly pathogenic avian influenza viruses in naive and vaccinated chickens. 2015.
Use and reproduction:
All rights reserved