High resolution genotyping of Campylobacter jejuni strains by macrorestriction analysis with Xho and polymerase chain reaction targeting enterobacterial repetitive intergenic consensus sequences: can we predict the zoonotic potential of strains?

Campylobacter jejuni isolates of human, canine, feline, bovine and poultry origin were investigated for their genomic diversity using O-antigen typing (n = 271), SmaI (n = 158) and XhoI (n = 158) macrorestriction analysis and ERIC-PCR (n = 107). The O-antigens 0: 1/44, 0: 2, 0:4 complex, 0: 37, 0:40 were identified and 53.7% of the human and 56.1% of the animal strains were typable with the available antisera. Two ERIC-PCR pattern groups were generated representing human and animal strains as well as those exclusively of animal origin. XhoI macrorestriction analysis also distinguished 'human' and 'non-human' strain clusters, but by Smal restriction mainly serotype-associated clusters were found. In conclusion, genomic differences may occur between 'human' and 'non-human' strains and this may reflect their potential to overcome the barrier from animals to humans