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Characterisation of cereal cyst nematodes in Egypt based on morphometrics, RFLP and rDNA-ITS sequence analyses

GND
1058939947
Zugehörigkeit
Julius Kühn-Institute (JKI), Institute for National and International Plant Health, Germany; Suez Canal University, Faculty of Agriculture, Agricultural Botany Department, Ismailia, Egypt
Baklawa, Mohamed Hassan Awad;
GND
123583926
Zugehörigkeit
Julius Kühn-Institute (JKI), Institute for National and International Plant Health, Germany
Niere, Björn;
GND
1058940058
Zugehörigkeit
Julius Kühn-Institute (JKI), Institute for Epidemiology and Pathogen Diagnostics, Germany
Heuer, Holger;
Zugehörigkeit
Suez Canal University, Faculty of Agriculture, Agricultural Botany Department, Ismailia, Egypt
Massoud, Samia

Morphological and molecular diversity among populations of cereal cyst nematodes (CCN) from wheat production areas in Ismailia province, Egypt, was investigated using light microscopy, ITS-RFLP and sequencing of the rDNA-ITS. CCN were found in five out of seven regions in Ismailia, the highest incidence being found in El Shark (West Sinai). The Egyptian populations were identified as H. avenae according to morphometrics of cyst vulval cone and second-stage juveniles. No differences in ITS-RFLP patterns generated by 17 restriction enzymes were detected among the Egyptian populations although the Egyptian populations could be distinguished from German populations of H. avenae and H. filipjevi. The analyses of ITS region sequences confirmed the species identification of the Egyptian populations as they clustered with H. avenae populations from Iran, Saudi Arabia, India, Israel and China.

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