Alternaria toxins of the alternariol type are not metabolised by human faecal microbiota

The metabolism of the Alternaria toxins alternariol (AOH), alternariol-9-O-methyl ether (AME) and altenuene (ALT) by the microbiota present in faeces from three human volunteers was studied. Faecal cultures were prepared as a 5% faeces suspension in brain-heart infusion broth and incubated with 50 μM of the toxins under anaerobic conditions for 72 h at 37 °C. The metabolism of AOH was also studied in pure bacterial cultures with either Escherichia coli DH5α or Lactobacillus plantarum BFE 5092 for 72 h at 37 °C. The three parent toxins were stable in uninoculated, heat-treated medium over a 72 h incubation period with a recovery of more than 90%. As a control for the activity of the faecal microbiota, the isoflavone daidzein was incubated with the faecal cultures and was transformed to its expected metabolites. In contrast, no metabolites of AOH, AME and ALT could be detected in the faecal cultures from the same volunteers, indicating that the gut microbiota was not capable of metabolising these substances. The Alternaria toxins could be shown to be at least partially bound to bacterial cells in a non-covalent manner, which may serve as a mechanism for their removal from the gut.