Clear distinction between Burkholderia mallei and Burkholderia pseudomallei using fluorescent motB primers

Schmoock, Gernot GND; Elschner, Mandy C. GND; Sprague, Lisa D. GND

Background A frame-shift mutation in the flagellum motor gene motB coding for the chemotaxis MotB protein of Burkholderia mallei has been utilized to design a conventional duplex PCR assay with fluorescent labelled primers. Findings Species specificity was tested with a panel of 13 Burkholderia type strains. A total of 41 B. mallei field strains, 36 B. pseudomallei field strains, and 1 B. thailandensis field strain from different geographic regions were tested and correctly identified. Testing of 55 non-Burkholderia bacterial species revealed 100% specificity of the assay. The minimum detection limit was 1 pg DNA or 160 GE for B. mallei and 130 GE for B. pseudomallei, respectively. Conclusions This assay enables the clear distinction between B. mallei and B. pseudomallei / B. thailandensis.

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Schmoock, Gernot / Elschner, Mandy C. / Sprague, Lisa D.: Clear distinction between Burkholderia mallei and Burkholderia pseudomallei using fluorescent motB primers. 2015.

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