Molecular marker-based identification of oilseed rape volunteers with different dormancy levels in oilseed rape

Pod dehiscence of oilseed rape (OSR) occurs before and during harvest, resulting in seed losses and in seed persistence in the soil for several years. Persisting seeds can emerge as volunteers in subsequent crops. Volunteers with deviating traits and especially genetically modified (GM) oilseed rape volunteers can impair the product quality of an oilseed rape crop. Under experimental field conditions seed persistence and volunteer abundance were found to be efficiently controlled by delayed post-harvest tillage and selection of cultivars with low secondary seed dormancy. To investigate the long-term factors affecting volunteer abundance in farmer-managed fields, an on-farm survey was carried out in winter oilseed rape fields at different locations in Germany, using artificial sowing gaps as one method to detect putative volunteers. Molecular marker analysis (ISSR-PCR), which was performed for 11 out of 31 fields, confirmed that most of the plants identified within sowing gaps were indeed volunteers. In fields with low OSR cropping frequency high percentages of volunteers could be assigned to previously cultivated varieties. Results indicate that seeds of varieties with high secondary dormancy (HD) persist in the fields for longer times and in larger numbers than seeds of varieties with low secondary dormancy. However, cultivation of HD varieties did not necessarily result in high volunteer abundance, as location and management factors have further impact on long-term seed survival. Nevertheless, with respect to GM oilseed rape cultivation proper variety selection will be an important means to assure co-existence with conventional oilseed rape.