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Genetic mapping of a leaf rust resistance gene in the former Yugoslavian barley landrace MBR1012

GND
1058993070
Affiliation
Julius Kuehn-Institute (JKI), Federal Research Centre for Cultivated Plants, Institute for Resistance Research and Stress Tolerance, Quedlinburg, Germany ; Department of Physiology and Biochemistry of Plants and Microbiology, Institute for Biology, Free University Berlin, Ko¨nigin-Luise-Str. 12-16, 14195 Berlin, Germany
König, Janine;
GND
1059141396
Affiliation
Julius Kuehn-Institute (JKI), Federal Research Centre for Cultivated Plants, Institute for Resistance Research and Stress Tolerance, Quedlinburg, Germany
Kopahnke, Doris;
Affiliation
Department of Plant Pathology, University of Minnesota, 495 Borlaug Hall, 1991 Upper Buford Circle, St. Paul, MN 55108-6030, USA
Steffenson, B. J.;
Affiliation
Scientific Institute of Field and Vegetable Crops Novi Sad, Maksima Gorkog 30, 21000 Novi Sad, Republic of Serbia
Przulj, N.;
Affiliation
Department of Physiology and Biochemistry of Plants and Microbiology, Institute for Biology, Free University Berlin, Ko¨nigin-Luise-Str. 12-16, 14195 Berlin, Germany
Romeis, T.;
Affiliation
Leibniz Institute of Plant Genetics and Crop Plant Research (IPK), Corrensstr. 3, 06466 Gatersleben, Germany
Röder, M. S.;
GND
172295300
Affiliation
Julius Kuehn-Institute (JKI), Federal Research Centre for Cultivated Plants, Institute for Resistance Research and Stress Tolerance, Quedlinburg, Germany
Ordon, Frank;
GND
1059141701
Affiliation
Julius Kuehn-Institute (JKI), Federal Research Centre for Cultivated Plants, Institute for Resistance Research and Stress Tolerance, Quedlinburg, Germany
Perovic, Dragan

Leaf rust, caused by Puccinia hordei Otth, is an important disease of barley (Hordeum vulgare L.) in many areas of the world. The appearance of new virulent races necessitates the identification of new resistance genes in barley. Screening of spring barley landraces from former Yugoslavia led to the identification of an accession (MBR1012) carrying resistance to the most widespread virulent leaf rust pathotypes in Europe. Ninety-one doubled haploid lines derived from a cross between landrace MBR1012 and the susceptible German cultivar Scarlett were evaluated for resistance to P. hordei isolate I-80 and segregated 48 resistant : 43 susceptible ( χ21:1=0.29, p = 0.6), indicating a monogenic inheritance of resistance. Using simple sequence repeats (SSR) and single nucleotide polymorphism (SNP) markers, the resistance gene in MBR1012 was mapped to the telomeric region of chromosome 1HS. This gene is assigned the temporary locus designation of Rph MBR1012 until it can be unequivocally determined to be different from all previously reported resistance genes. The closest flanking markers for Rph MBR1012 are located 0.8 cM distal (SNP marker GBS546 and SSR marker GBMS187) and 6.0 cM proximal (SSR marker GMS21). The diagnostic value of the closest linked markers was assessed in a genetically diverse collection of 51 susceptible and resistant barley lines and cultivars. The SSR GBMS187 predicted the presence of Rph MBR1012 with 100% accuracy. However, this marker could not be used singly for the rapid incorporation of resistance into high yielding barley cultivars, since it detects a null allele in MBR1012. Therefore, simultaneous use of the markers closely linked to Rph MBR1012 is needed for transferring Rph MBR1012 into adapted cultivars.

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