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Development of a cDNA-AFLP derived CAPS marker co-segregating with the powdery mildew resistance gene Mlg in barley

Zugehörigkeit
Institute of Phytopathology and Applied Zoology, Justus-Liebig-University, Heinrich-Buff-Ring 26–32, D-35392 Giessen, Germany
Korell, M.;
Zugehörigkeit
Institute of Phytopathology and Applied Zoology, Justus-Liebig-University, Heinrich-Buff-Ring 26–32, D-35392 Giessen, Germany
Eschholz, T. W.;
Zugehörigkeit
Institute of Phytopathology and Applied Zoology, Justus-Liebig-University, Heinrich-Buff-Ring 26–32, D-35392 Giessen, Germany
Eckey, C.;
Zugehörigkeit
Institute of Phytopathology and Applied Zoology, Justus-Liebig-University, Heinrich-Buff-Ring 26–32, D-35392 Giessen, Germany
Biedenkopf, D.;
Zugehörigkeit
Institute of Phytopathology and Applied Zoology, Justus-Liebig-University, Heinrich-Buff-Ring 26–32, D-35392 Giessen, Germany
Kogel, Marc K.-H.;
GND
143673491
Zugehörigkeit
Institute of Crop Science and Plant Breeding I, Justus-Liebig-University, Heinrich-Buff-Ring 26–32, D-35392 Giessen, Germany
Friedt, Wolfgang;
GND
172295300
Zugehörigkeit
Federal Centre for Breeding Research on Cultivated Plants, Institute of Epidemiology and Resistance Resources, Quedlinburg, Germany
Ordon, Frank

Powdery mildew is an important pathogen of barley in many barleygrowing areas. To identify molecular markers for the resistance gene Mlg located on chromosome 4H, a cDNA-AFLP approach was carried out on near-isogenic lines. Based on the identification of a differentiating fragment of 37 bp, which turned out to be part of a nucleoside diphosphate kinase as estimated by BLASTN, a CAPS marker has been developed, which co-segregated with Mlg in a population of 738 F2-plants. Due to its co-dominant inheritance, clear banding pattern and close linkage this marker is well suited for marker-based selection procedures.

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