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Regulation of gene expression by chromosome 5A during cold hardening in wheat

Zugehörigkeit
Agricultural Research Institute of the Hungarian Academy of Sciences, Brunszvik u. 2, Martonvásár 2462, Hungary ; Department Genebank, AG Genome Diversity (GED), Leibniz Institute of Plant Genetics and Crop Plant Research (IPK), Corrensstrasse 3, 06466 Gatersleben, Germany
Kocsy, Gábor;
Zugehörigkeit
Department Genebank, AG Genome Diversity (GED), Leibniz Institute of Plant Genetics and Crop Plant Research (IPK), Corrensstrasse 3, 06466 Gatersleben, Germany
Athmer, Benedikt;
GND
1059141701
Zugehörigkeit
Julius Kuehn-Institute (JKI), Federal Research Centre for Cultivated Plants, Institute for Resistance Research and Stress Tolerance, Quedlinburg, Germany ; Department Genebank, AG Genome Diversity (GED), Leibniz Institute of Plant Genetics and Crop Plant Research (IPK), Corrensstrasse 3, 06466 Gatersleben, Germany
Perovic, Dragan;
Zugehörigkeit
Department Genebank, AG Genome Diversity (GED), Leibniz Institute of Plant Genetics and Crop Plant Research (IPK), Corrensstrasse 3, 06466 Gatersleben, Germany
Himmelbach, Axel;
Zugehörigkeit
Agricultural Research Institute of the Hungarian Academy of Sciences, Brunszvik u. 2, Martonvásár 2462, Hungary
Szücs, Attila;
Zugehörigkeit
Agricultural Research Institute of the Hungarian Academy of Sciences, Brunszvik u. 2, Martonvásár 2462, Hungary ; Faculty of Information Technology, Research Institute of Chemical and Process Engineering, University of Pannonia, Egyetem u. 10, Veszprém 8200, Hungary
Vashegyi, Ildikó;
Zugehörigkeit
Department Genebank, AG Genome Diversity (GED), Leibniz Institute of Plant Genetics and Crop Plant Research (IPK), Corrensstrasse 3, 06466 Gatersleben, Germany
Schweizer, Patrick;
Zugehörigkeit
Agricultural Research Institute of the Hungarian Academy of Sciences, Brunszvik u. 2, Martonvásár 2462, Hungary ; Faculty of Information Technology, Research Institute of Chemical and Process Engineering, University of Pannonia, Egyetem u. 10, Veszprém 8200, Hungary
Galiba, Gábor;
Zugehörigkeit
Department Genebank, AG Genome Diversity (GED), Leibniz Institute of Plant Genetics and Crop Plant Research (IPK), Corrensstrasse 3, 06466 Gatersleben, Germany
Stein, Nils

Cold hardening is necessary to achieve the genetically determined maximum freezing tolerance and to reduce yield losses in winter cereals. The aim of the present study was to determine a set of genes with an important role in this process, by comparing of chromosome 5A substitution lines with different levels of freezing tolerance, since chromosome 5A is a major regulator of this trait. During 21 days of treatment at 2C, 303 genes were upregulated, while 222 were down-regulated at most sampling points, and 156 at around half of them (out of the 10,297 unigenes studied). The freezing-tolerant substitution line exhibited 1.5 times as many differentially expressed genes than the sensitive one. The transcription of 78 genes (39 up-regulated) proved to be chromosome 5Adependent. These genes encoded proteins involved in transcriptional regulation, defence processes and carbohydrate metabolism. Three of the chromosome 5A-related genes, coding for a cold-responsive, a Ca-binding and an embryo and meristem-related protein, were genetically mapped and characterized in further detail. The present experimental system was appropriate for the selection of chromosome 5A-related genes involved in short- and long-term cold acclimation in wheat. By modifying the expression of these genes it may be possible to improve freezing tolerance.

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