Article All rights reserved
refereed
published

PhyloChip hybridisation uncovered an enormous bacterial diversity in the rhizosphere of different potato cultivars: many common and few cultivar-dependent taxa

GND
1186945443
Affiliation
Julius Kühn-Institute (JKI), Federal Research Centre for Cultivated Plants, Institute for Epidemiology and Pathogen Diagnostics, Brunswick, Germany
Weinert, Nicole;
Affiliation
Lawrence Berkeley National Laboratory, Berkeley, CA, USA
Piceno, Yvette;
GND
102059845X
Affiliation
Julius Kühn-Institute (JKI), Federal Research Centre for Cultivated Plants, Institute for Epidemiology and Pathogen Diagnostics, Brunswick, Germany
Ding, Guo-Chun;
Affiliation
Graz University of Technology, Environmental Biotechnology, Graz, Austria
Meincke, Remo;
GND
1058940058
Affiliation
Julius Kühn-Institute (JKI), Federal Research Centre for Cultivated Plants, Institute for Epidemiology and Pathogen Diagnostics, Brunswick, Germany
Heuer, Holger;
Affiliation
Graz University of Technology, Environmental Biotechnology, Graz, Austria
Berg, Gabriele;
Affiliation
Helmholtz Zentrum München, Department for Terrestrial Ecogenetics, Oberschleissheim, Germany
Schloter, Michael;
Affiliation
Lawrence Berkeley National Laboratory, Berkeley, CA, USA
Andersen, Gary;
GND
1058967878
Affiliation
Julius Kühn-Institute (JKI), Federal Research Centre for Cultivated Plants, Institute for Epidemiology and Pathogen Diagnostics, Brunswick, Germany
Smalla, Kornelia

The phylogenetic composition of bacterial communities in the rhizosphere of three potato cultivars grown at two distant field sites was analysed. Ribosomal gene fragments amplified fromtotal community DNAwere hybridized to PhyloChips. A total of 2432 operational taxonomic units (OTUs) were detected by the Phylo- Chips, of which 65% were found in the rhizosphere of all cultivars at both field sites. From all detected OTUs, 9% revealed a cultivar-dependent abundance at the one or the other field site and 4% at both sites. Differential abundance on the three cultivars was mainly observed for OTUs belonging to the Pseudomonadales, Actinomycetales and Enterobacteriales. More than 40% of OTUs belonging to Bradyrhizobiales, Sphingomonadales, Burkholderiales, Rhodocyclales, Xanthomona- dales and Actinomycetales differed significantly in their abundance between the sites. A sequence analysis of six 16S rRNA gene clone libraries corresponded well with the taxonomic community structure evidenced by the PhyloChip hybridiza- tion. Most ribotypes matched OTUs detected by the PhyloChip. Those OTUs that responded to the potato cultivar at both field sites might be of interest in view of cultivar-specific effects on bacterial biocontrol strains and pathogens.

Files

Cite

Citation style:
Could not load citation form.

Access Statistic

Total:
Downloads:
Abtractviews:
Last 12 Month:
Downloads:
Abtractviews:

Rights

Use and reproduction:
All rights reserved