Prevalence of Extended-Spectrum beta-Lactamases producing Escherichia coli on Bavarian dairy and beef cattle farms

Schmid, A.; Hormansdorfer, S.; Messelhäußer, U.; Käsbohrer, A.; Sauter-Louis, C.; Mansfeld, R.

Extended-spectrum beta-lactamase producing Escherichia coli (ESBL E. coli) are believed to be widely distributed among humans and animals; however to date, there are only few studies that support this assumption on a regional or country-wide scale. Therefore, a study was designed to assess the prevalence of ESBL E. coli in dairy cows and beef cattle in the southern part of Bavaria, Germany. The study population included 30 mixed dairy and beef cattle farms and 15 beef cattle farms. Fecal samples, boot swabs and dust samples were analyzed for ESBL E. coli using selective media. PCR was performed to screen for CTX-M and ampC resistance genes. A total of 598 samples yielded 196 (32.8%) ESBL E. coli, originating from 39 (86.7%) of 45 farms. Samples obtained from mixed farms were significantly more likely to be ESBL E. coli positive than samples from beef cattle farms (fecal samples p<0.001, boot swabs p=0.014 and dust samples p=0.041). A total of 183 isolates (93.4%) of 196 ESBL E. coli positive strains harbored CTX-M genes, CTX-M group 1 being the most frequently found group. Forty six additional isolates contained ampC genes and five of the 46 isolates expressed a blaCMY-2 gene. The study shows that ESBL E. coli are commonly found on Bavarian dairy and beef cattle farms. Moreover, to our knowledge, this is the first report of the occurrence of blaCMY-2 in cattle in Germany

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Schmid, A. / Hormansdorfer, S. / Messelhäußer, U. / et al: Prevalence of Extended-Spectrum beta-Lactamases producing Escherichia coli on Bavarian dairy and beef cattle farms. 2013.

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