Rapid Spoligotyping of Mycobacterium tuberculosis Complex Bacteria by Use of a Microarray System with Automatic Data Processing and Assignment

Rüttger, Anke; Nieter, Johanna; Skrypnyk, A.; Engelmann, I.; Ziegler, A.; Moser, Irmgard; Monecke, S.; Ehricht, R.; Sachse, Konrad

doi:10.1128/JCM.0042-12

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Rapid Spoligotyping of Mycobacterium tuberculosis Complex Bacteria by Use of a Microarray System with Automatic Data Processing and Assignment

Rüttger, Anke ; Nieter, Johanna ; Skrypnyk, A.; Engelmann, I.; Ziegler, A.; Moser, Irmgard ; Monecke, S.; Ehricht, R.; Sachse, Konrad

Membrane-based spoligotyping has been converted to DNA microarray format to qualify it for high-throughput testing. We have shown the assay's validity, as well as its suitability for direct typing from tissue and detecting new spoligotypes. Advantages of the microarray methodology include rapidity, ease of operation, automatic data processing and affordability.

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Erschienen in:: Journal of clinical microbiology
Vol. 50, H. 7 (2012), S. 2492-2495
Band:: 50
Heft:: 7
Datum der Veröffentlichung:: 02.05.2012
DOI:: 10.1128/JCM.0042-12
Sprache:: Englisch
Ressourcentyp:: Text
Schlagwörter:: Mycobacterium tuberculosis complex; spoligotyping; SpolDB4.0; Mbovis.org; DNA microarray
DDC-Sachgruppe der DNB:: 570 Biowissenschaften, Biologie
Link URL:: http://jcm.asm.org/content/50/7/2492.long
Link URL:: http://www.ncbi.nlm.nih.gov/pmc/articles/PMC3405571/
Einrichtung:: Friedrich-Loeffler-Institut, Institut für Molekulare Pathogenese
Physischer Standort:: SD/2012/197