Simple, Sensitive, and Swift Sequencing of Complete H5N1 Avian Influenza Virus Genomes

Höper, Dirk; Hoffmann, Bernd; Beer, Martin

doi:10.1128/JCM.01028-08

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Simple, Sensitive, and Swift Sequencing of Complete H5N1 Avian Influenza Virus Genomes

Höper, Dirk ; Hoffmann, Bernd ; Beer, Martin

The spread of highly pathogenic avian influenza A virus (HPAIV) of subtype H5N1 demands fast and reliable methods for in-depth, full-length sequence analysis. For this purpose, we designed a simple and sensitive method for the preparation of sequencing libraries from H5N1 HPAIV diagnostic RNA samples for sequencing with the Genome Sequencer FLX instrument. The method presented seamlessly integrates high-throughput pyrosequencing with the Roche/454 instrument into diagnostics without the need for additional equipment or molecular biological techniques besides standard PCR and the Genome Sequencer FLX sample preparation and sequencing pipeline

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Erschienen in:: Journal of clinical microbiology
Vol. 47, H. 3 (2009), S. 674-679
Band:: 47
Heft:: 3
Datum der Veröffentlichung:: 24.12.2008
DOI:: 10.1128/JCM.01028-08
Sprache:: Englisch
Ressourcentyp:: Text
Schlagwörter:: A VIRUS; avian; Avian influenza; Avian influenza virus; diagnostics; GENOME; GERMANY; H5N1; HIGH-THROUGHPUT; highly pathogenic; Highly pathogenic avian influenza; HPAIV; Influenza A virus; influenza virus; methods; microbiology; PCR; SEQUENCE; SEQUENCE-ANALYSIS; sequencing; spread; SUBTYPE H5N1; VIRUS
DDC-Sachgruppe der DNB:: 630 Landwirtschaft, Veterinärmedizin
Link URL:: http://jcm.asm.org/content/47/3/674.long
Link URL:: http://www.ncbi.nlm.nih.gov/pmc/articles/PMC2650901/
Einrichtung:: Friedrich-Loeffler-Institut, Institut für Virusdiagnostik
Physischer Standort:: SD/2009/76