Rapid and Highly Sensitive Neuraminidase Subtyping of Avian Influenza Viruses by Use of a Diagnostic DNA Microarray

Gall, Astrid; Hoffmann, Bernd; Harder, Timm C.; Grund, Christian; Ehricht, Ralf; Beer, Martin

doi:10.1128/JCM.00850-09

referiert

Veröffentlicht

Rapid and Highly Sensitive Neuraminidase Subtyping of Avian Influenza Viruses by Use of a Diagnostic DNA Microarray

Gall, Astrid ; Hoffmann, Bernd ; Harder, Timm C.; Grund, Christian ; Ehricht, Ralf; Beer, Martin

Rapid neuraminidase subtyping of avian influenza viruses from diagnostic samples is crucial considering the existence of permanently emerging and evolving strains. Here we report an easy-to-use, low-cost microarray for neuraminidase subtyping following fragment amplification by a generic, neuraminidase-specific reverse transcription-PCR (RT-PCR). This method enables highly specific characterization with a sensitivity equal to that of matrix gene-specific real-time RT-PCR

Vorschau

Einordnung

Erschienen in:: Journal of clinical microbiology
Vol. 47, H. 9 (2009), S. 2985-2988
Band:: 47
Heft:: 9
Datum der Veröffentlichung:: 08.07.2009
DOI:: 10.1128/JCM.00850-09
Sprache:: Englisch
Ressourcentyp:: Text
Schlagwörter:: A VIRUSES; AMPLIFICATION; avian; Avian influenza; DNA; GENE; GERMANY; microbiology; Neuraminidase; REAL-TIME; real-time RT-PCR; REVERSE-TRANSCRIPTION-PCR; RT-PCR; sensitivity; STRAIN; STRAINS; Subtyping; UNIVERSAL PRIMER SET; VIRUSES
DDC-Sachgruppe der DNB:: 570 Biowissenschaften, Biologie
Link URL:: http://jcm.asm.org/content/47/9/2985.long
Link URL:: http://www.ncbi.nlm.nih.gov/pmc/articles/PMC2738067/
Einrichtung:: Friedrich-Loeffler-Institut, Institut für Virusdiagnostik
Physischer Standort:: SD/2009/210