Analysis of blaCTX-M-carrying plasmids from Escherichia coli isolates collected in the BfT-GermVet study

In this study, 417 E. coli from defined disease conditions of companion and farm animals collected in the BfT-GermVet study were investigated for the presence of extended spectrum beta-lactamase (ESBL) genes. Three ESBL-producing E. coli isolates were identified among the 100 ampicillin-resistant isolates. The E. coli isolates 168 and 246 of canine and porcine origin, respectively, harboured bla(CTX-M-1) and the canine isolate 913 bla(CTX-M-15) as confirmed by PCR and sequence analysis. The isolates 168 and 246 belonged to the novel MLST types ST1576 and ST1153, respectively, while isolate 913 had the MLST type ST410. The ESBL genes were located on structurally related IncN plasmids in isolates 168 and 246 and on an IncF plasmid in isolate 913. The bla(CTX-M-1) upstream regions of plasmids pCTX168 and pCTX246 were similar whereas the downstream regions showed structural differences. The genetic environment of the bla(CTX-M-15) gene on plasmid pCTX913 differed distinctly from that of both bla(CTX-M-1) genes. Detailed sequence analysis showed that the integration of insertion sequences as well as interplasmid recombination events accounted for the structural variability in the bla(CTX-M) gene regions



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