Effect of rare earth elements on beef cattle growth performance, blood clinical chemical parameters and mitogen stimulated proliferation of bovine peripheral blood mononuclear cells in vitro and ex vivo

Rare earth elements (REE) are possible performance enhancers in animal production, but little is known about their effects on ruminants. Therefore a feeding trial was conducted with 40 fattening bulls who received 0, 100, 200 or 300 mg REE-citrate/kg dry matter (DM), containing 34.30% La, 58.09% Ce and 7.61% other REE. DM intake was measured daily and live weight weekly. Ex vivo ConcanavalinA (ConA)-stimulated cell proliferation of peripheral bood mononuclear cells (PBMC) was tested by MTT and alamar blue (AB) assay. Serum was analysed for clinical chemical parameters, ion (Mg, Ca and P) and REE concentrations. The effects of LaCl(3), CeCl(3), NdCl(3) and YCl(3) on ConA-stimulated proliferation of PBMC were tested in vitro, using MTT and AB assay. REE-citrat supplementation did not affect DM intake, live weight gain, clinical chemical parameters, and ion concentrations significantly. In REE-300 group ex vivo proliferation of PBMC was significantly increased. In vitro ConA-stimulated proliferation decreased with rising REE-chlorid concentrations. At least at the highest tested concentration (approximately 290 muM) the inhibition reached significance. Proliferation of non-stimulated PBMC was not affected dose-dependently. REE affect the proliferation of PBMC, thus an effect on the bovine immune system is possible. However, the great differences in effective doses in vitro and ex vivo (serum REE concentrations) might explain the different results from the experiments