A potential novel Brucella species isolated from mandibular lymph nodes of red foxes in Austria

Hofer, E.; Revilla-Fernandez, S.; Al Dahouk, S.; Riehm, J.M.; Nöckler, K.; Zygmunt, M.S.; Cloeckaert, A.; Tomaso, Herbert; Scholz, H.C.

doi:10.1016/j.vetmic.2011.08.009

referiert

Veröffentlicht

A potential novel Brucella species isolated from mandibular lymph nodes of red foxes in Austria

Hofer, E.; Revilla-Fernandez, S.; Al Dahouk, S.; Riehm, J.M.; Nöckler, K.; Zygmunt, M.S.; Cloeckaert, A.; Tomaso, Herbert ; Scholz, H.C.

The wild red fox (Vulpes vulpes) is a known indicator species for natural foci of brucellosis. Here, we describe phenotypic and molecular characteristics of two atypical Brucella strains isolated from two foxes hunted 2008 in Eastern Austria. Both strains agglutinated with monospecific anti-Brucella A serum and were positive in ELISA with monoclonal antibodies directed against various Brucella lipopolysaccharide epitopes. However, negative nitrate reductase- and negative oxidase-reaction were atypical traits. Affiliation to the genus Brucella was confirmed by 16S rRNA gene sequencing and by detection of the Brucella specific insertion element IS. 711 and gene bcsp31 using real-time PCR. Both fox strains showed identical IS. 711 Southern blot profiles but were distinct from known brucellae. The number of IS. 711 copies detected was as high as found in B. ovis or marine mammal Brucella strains. Molecular analyses of the recA and omp2a/b genes suggest that both strains possibly represent a novel Brucella species.

Dateien

beschränkter Zugriff

Einordnung

Erschienen in:: Veterinary microbiology
Vol. 155, H. 1 (2012), S. 93-99
Band:: 155
Heft:: 1
Datum der Veröffentlichung:: 16.08.2011
DOI:: 10.1016/j.vetmic.2011.08.009
Sprache:: Englisch
Ressourcentyp:: Text
Schlagwörter:: 16S rRNA gene; 3; ABORTUS; AGE; Al; Amino Acid Sequence; Analyse; Animals; Antibodies; ANTIBODY; article; ASSAY; assessment; Austria; bacterial gene; Bacterial Infections; bacterial RNA; bacterial strain; Base Sequence; Berlin; Brucella; Brucella abortus; Brucella canis; Brucella ceti; Brucella inopinata; Brucella melitensis; brucella neotomae; Brucella ovis; Brucella pinnipedialis; Brucella suis; Brucellosis; Canidae; characterisation; characterization; classification; CLUSTER; control; detection; development; diagnosis; DIFFERENTIATION; DISEASE; DNA; ELISA; English Abstract; enzyme linked immunosorbent assay; Enzyme-Linked Immunosorbent Assay; evaluation; Flow Cytometry; Foci; Food; food safety; fox; Foxes; France; gene; gene cluster; gene dosage; gene sequence; GENES; genome; GENOME SEQUENCE; genomic; Genotyping; german; Germany; HEALTH; IDENTIFICATION; In Vitro; IN-VITRO; INFECTION; INFECTIONS; isolation; Laboratories; Lipopolysaccharide; Livestock; LOCUS; Lymph node; Lymph Nodes; Mammalia; MAMMALS; mandibular lymph node; marine; MARINE MAMMALS; MARKERS; Membrane Proteins; MEMBRANE-PROTEIN; method; methods; Mice; microbiology; molecular characterisation; MOLECULAR CHARACTERIZATION; Molecular Sequence Data; Monoclonal antibodies; MULTIPLEX PCR; multiplex-PCR; natural; NATURAL FOCI; new species
DDC-Sachgruppe der DNB:: 630 Landwirtschaft, Veterinärmedizin
Link URL:: http://www.sciencedirect.com/science/article/pii/S037811351100455X
Einrichtung:: Bundesinstitut für Risikobewertung, Bundesinstitut für Risikobewertung (bis Juni 2014), Abteilung 7 - Produktsicherheit (bis Juni 2014), Fachgruppe 74 - Hygiene und Mikrobiologie
Einrichtung:: Bundesinstitut für Risikobewertung, Bundesinstitut für Risikobewertung (bis Juni 2014), Abteilung 4 - Biologische Sicherheit (bis Juni 2014)
Einrichtung:: Bundesinstitut für Risikobewertung, Bundesinstitut für Risikobewertung (bis Juni 2014), Abteilung 4 - Biologische Sicherheit (bis Juni 2014), Fachgruppe 45 - Diagnostik, Genetik und Erregercharakterisierung
Einrichtung:: Friedrich-Loeffler-Institut, Institut für Bakterielle Infektionen und Zoonosen
Physischer Standort:: SD/2012/21