Rotavirus RNA polymerases resolve into two phylogenetically distinct classes that differ in their mechanism of template recognition

Ogden,K.M.; Johne,R.; Patton,J.T.

doi:10.1016/j.virol.2012.05.011

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Rotavirus RNA polymerases resolve into two phylogenetically distinct classes that differ in their mechanism of template recognition

Rotaviruses (RVs) are segmented double-stranded RNA viruses that cause gastroenteritis in mammals and birds. Within the RV genus, eight species (RVA-RVH) have been proposed. Here, we report the first RVF and RVG sequences for the viral RNA polymerase (VP1)-encoding segments and compare them to those of other RV species. Phylogenetic analyses indicate that the VP1 RNA segments and proteins resolve into two major clades, with RVA, RVC, RVD and RVF in clade A, and RVB, RVG and RVH in clade B. Plus-strand RNA of clade A viruses, and not clade B viruses, contain a 3'-proximal UGUG cassette that serves as the VP1 recognition signal. VP1 structures for a representative of each RV species were predicted using homology modeling. Structural elements involved in interactions with the UGUG cassette were conserved among VP1 of clade A, suggesting a conserved mechanism of viral RNA recognition for these viruses.

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Erschienen in:: Virology
Vol. 431, H. 1-2 (2012), S. 50-57
Band:: 431
Heft:: 1-2
Datum der Veröffentlichung:: 2012
DOI:: 10.1016/j.virol.2012.05.011
Sprache:: Englisch
Ressourcentyp:: Text
Schlagwörter:: 3' untranslated region; A VIRUSES; Adult; algorithm; allergy; Analyse; analysis; Animals; article; assessment; Aves; Bangladesh; Berlin; Birds; bovine; broiler; broiler chicken; Broiler Chickens; BROILER-CHICKENS; Cercopithecine herpesvirus 1; characterization; chicken; Chickens; cladistics; classification; Cluster Analysis; COMPLEX; COMPLEXES; controlled study; diarrhoea; DISEASE; DISEASES; DNA-Directed RNA Polymerases; double stranded RNA; Double-stranded RNA virus; epidemiology; Evolution; Gastroenteritis; gene; gene cassette; GENES; genetic conservation; genome; GENOME SEQUENCE; genomic; Genotype; Germany; guanine; HEALTH; HOMOLOGY; Human; IDENTIFICATION; INFECTION; infectious disease; initiation; international; Laboratories; Mammalia; MAMMALS; MECHANISM; model; Models,Molecular; MOLECULAR CHARACTERIZATION; molecular mechanics; molecular phylogeny; molecular recognition; Molecular Sequence Data; nonhuman; nucleotide sequence; NUMBER; NUMBERS; ORIGIN; Phylogeny; Polymerase structure; Prediction; priority journal; protein; protein conformation; protein protein interaction; protein structure; protein VP1; Proteins; recognition; reference; Reovirus; Report; Research; Risk; Risk Assessment; RISK-ASSESSMENT; RNA polymerase; RNA sequence; RNA virus; RNA viruses; Rna,Viral; rotavirus; Rotavirus A; Rotavirus B; Rotavirus C; Rotavirus D; Rotavirus F; Rotavirus G; Rotavirus H; Rotavirus infection; Rotaviruses; SEQUENCE; sequence analysis; Sequence Analysis,DNA; Sequence Homology; SEQUENCE-ANALYSIS; SEQUENCES; SIGNAL; Signal Transduction; species; species comparison; STATE; STRAIN; STRAINS; SYSTEM; Templates,Genetic; United States; UNITED-STATES; uridine; values; Viral Core Proteins; virology; VIRUSES; VP1; VP4; VP6; RNA; mammal; bird
DDC-Sachgruppe der DNB:: 630 Landwirtschaft, Veterinärmedizin
Link URL:: http://www.sciencedirect.com/science/article/pii/S0042682212002449
Einrichtung:: Bundesinstitut für Risikobewertung, Bundesinstitut für Risikobewertung (bis Juni 2014), Abteilung 4 - Biologische Sicherheit (bis Juni 2014), Fachgruppe 42 - Lebensmittelhygiene und Sicherheitskonzepte