Field trial of six serological tests for bovine brucellosis

Abernethy, D. A.; Menzies, F. D.; McCullough, S. J.; McDowell, S. W. J.; Burns, K. E.; Watt, R.; Gordon, A. W.; Greiner, M.; Pfeiffer, D. U.

doi:10.1016/j.tvjl.2011.03.008

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Field trial of six serological tests for bovine brucellosis

Abernethy, D. A.; Menzies, F. D.; McCullough, S. J.; McDowell, S. W. J.; Burns, K. E.; Watt, R.; Gordon, A. W.; Greiner, M.; Pfeiffer, D. U.

Serum agglutination (SAT), complement fixation (CFT), indirect ELISA (iELISA), competitive ELISA (cELISA), Rose Bengal (RBT) and EDTA-modified agglutination (EDTA) tests were used in parallel on serological samples from 19,935 cattle in 301 herds. The study herds were selected according to putative exposure to Brucella abortus with cases defined by bacteriological culture or test agreement. No single test identified all infected cattle and, at diagnostic thresholds, relative sensitivity was highest in the iELISA (67.9%) or RBT (78.1%), using bacteriological culture or test agreement, respectively, to define cases. As screening tests, the relative sensitivity of the SAT was highest (75.9% by culture or 84.9% by test agreement), with an optimal threshold of 31 IU. The relative specificity of the diagnostic tests ranged from 99.6% (SAT 31 IU) to 100% (iELISA, RBT and CFT). The trial confirmed the value of the SAT as a screening test and the value of parallel testing.

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Erschienen in:: The Veterinary Journal
Vol. 191, H. 3 (2012), S. 364-370
Band:: 191
Heft:: 3
Datum der Veröffentlichung:: 2012
DOI:: 10.1016/j.tvjl.2011.03.008
Sprache:: Englisch
Ressourcentyp:: Text
Schlagwörter:: ABORTUS; ACID; agglutination test; Agglutination Tests; Agriculture; animal; Animals; Antibodies; ANTIBODY; article; assessment; ASSOCIATION; Australia; Authorities; bacterium culture; Berlin; blood; Bos; Bovinae; bovine; BOVINE BRUCELLOSIS; Brucella; Brucella abortus; Brucella melitensis biovar Abortus; Brucellosis; Brucellosis,Bovine; Canada; Cattle; comparative; comparison; competitive enzyme linked immunosorbent assay; complement fixation test; Complement Fixation Tests; controlled study; COWS; CULTURE; DAIRY; detection; development; diagnosis; diagnostic; Diagnostic tests; diagnostic value; economics; Edetic Acid; EDTA modified agglutination test; EFSA; ELISA; ENZYME; enzyme linked immunosorbent assay; ENZYME-IMMUNOASSAY; Enzyme-Linked Immunosorbent Assay; epidemiology; eradication; European food safety authorities; evaluation; exposure; Food; food safety; Germany; goat; Goats; herd; HERDS; hygiene; Immunoassay; immunology; immunopathology; indirect enzyme linked immunosorbent assay; INFECTION; international; ISSUES; Laboratories; MANAGEMENT; method; methods; microbiology; MILK; NO; nonhuman; Northern Ireland; PERFORMANCE; PERSISTENCE; reduction; reference; Report; Research; review; Risk; Risk Assessment; RISK-ASSESSMENT; rose bengal; rose bengal test; Safety; SAMPLES; SCIENCE; screening; SENSITIVITY; Sensitivity and Specificity; SERA; Serologic Tests; Serology; serum; serum agglutination test; Sheep; SINGLE; SPECIFICITY; standard; standards; studies; study; Symposium; technique; Test accuracy; test performance; Testing; TESTS; TRIAL; United Kingdom; UNITED-KINGDOM; VACCINE; vaccines; validation; veterinary; Veterinary Medicine; VETERINARY-MEDICINE
DDC-Sachgruppe der DNB:: 570 Biowissenschaften, Biologie
Link URL:: http://www.sciencedirect.com/science/article/pii/S1090023311001110
Einrichtung:: Bundesinstitut für Risikobewertung, Bundesinstitut für Risikobewertung (bis Juni 2014), Abteilung 3 - Wissenschaftliche Querschnittsaufgaben (bis Juni 2014)