Clonal dissemination of Salmonella enterica serovar Infantis in Germany

Hauser,E.; Tietze,E.; Helmuth,R.; Junker,E.; Prager,R.; Schroeter,A.; Rabsch,W.; Fruth,A.; Toboldt,A.; Malorny,B.

doi:10.1089/fpd.2011.1038

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Clonal dissemination of Salmonella enterica serovar Infantis in Germany

Hauser,E.; Tietze,E.; Helmuth,R.; Junker,E.; Prager,R.; Schroeter,A.; Rabsch,W.; Fruth,A.; Toboldt,A.; Malorny,B.

Salmonella enterica serovar Infantis (Salmonella Infantis) is consistently isolated from broiler chickens, pigs, and humans worldwide. This study investigated 93 epidemiologically unrelated Salmonella Infantis strains isolated in Germany between 2005 and 2008 in respect to their transmission along the food chain. Various phenotypic and genotypic methods were applied, and the pathogenicity and resistance gene repertoire was determined. Phenotypically, 66% of the strains were susceptible to all 17 antimicrobials tested, while the others were almost all multidrug-resistant (two or more antimicrobial resistances), with different resistance profiles and preferentially isolated from broiler chickens. A number of phage types (PTs) were shared by strains from pigs, broiler chickens, and humans (predominated by PT 29). One, PT 1, was only detected in strains from pigs/pork and humans. Pulsed-field gel electrophoresis (PFGE) subdivided strains in seven different clusters, named A-G, consisting of 35 various XbaI profiles with coefficient of similarity values of 0.73-0.97. The majority of XbaI profiles were assigned to clusters A and C, and two predominant XbaI profiles were common in strains isolated from all sources investigated. Multi-locus sequence typing (MLST) analysis of selected strains representing the seven PFGE clusters revealed that they all belonged to ST32. The pathogenicity gene repertoire of 37 representative Salmonella Infantis strains analyzed by microarray was also identical. The resistance gene repertoire correlated perfectly with the phenotypic antimicrobial resistance profiles, and multidrug-resistant strains were associated with class 1 integrons. Overall, this study showed that two major closely related genotypes of Salmonella Infantis can transmit in Germany to humans through contaminated broiler meat or pork, and consequently presents a hazard for human health. -® Copyright 2012, Mary Ann Liebert, Inc. 2012

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Erschienen in:: Foodborne pathogens and disease
Vol. 9, H. 4 (2012), S. 352-360
Band:: 9
Heft:: 4
Datum der Veröffentlichung:: 2012
DOI:: 10.1089/fpd.2011.1038
Sprache:: Englisch
Ressourcentyp:: Text
Schlagwörter:: 3; 37; AGENT; AGENTS; analysis; animal; Animals; antibiotic; antibiotic resistance; antibiotic sensitivity; ANTIBIOTIC-RESISTANCE; ANTIMICROBIAL RESISTANCE; article; assessment; Authorities; Bacteria; bacterial strain; bacterial transmission; bacteriophage; bacterium; Berlin; biology; Biotechnology; bovine; BRAZIL; broiler; broiler chicken; Broiler Chickens; BROILER-CHICKENS; BROILERS; CALIFORNIA; characterisation; characterization; chicken; CHICKEN MEAT; Chickens; class 1 integrons; class 2 integrons; clinical; CLUSTER; COLI; COLLECTION; COMMUNITY; comparison; control; COUNTRIES; criteria; data collection; development; diagnostic; Dilution; DISEASE; DISEASES; distribution; DIVERSITY; DNA; EC; EFSA; Electrophoresis; EMERGENCE; ENTERICA; ENTERICA SEROVAR TYPHIMURIUM; ENVIRONMENT; epidemiology; Escherichia coli; ESCHERICHIA-COLI; EU; Europe; European Union; EUROPEAN-UNION; farm; flocks; Food; Food Chain; Food Microbiology; food safety; food-borne outbreaks; FOOD-CHAIN; foodborne infection; Gallus gallus; gel; gel electrophoresis; GEL-ELECTROPHORESIS; gene; GENES; genome; Genotype; GENOTYPES; Germany; hazard; HEALTH; Human; HUMAN HEALTH; Humans; hygiene; immunology; IMPACT; INFECTION; INFECTIONS; integron; Integrons; international; investigation; Italy; Japan; Laboratories; line; Longitudinal Studies; longitudinal study; Meat; method; methods; microarray; microarray analysis; microbiology; MLST; molecular characterisation; molecular epidemiology; molecular typing; monitoring; multidrug resistance; multilocus sequence typing; MULTIPLE-DRUG RESISTANCE; NO; nonhuman; NUMBER; ORGANIZATION; OUTBREAK; OUTBREAKS; Paratyphi; PARATYPHI-B; pathogenicity; PATTERN; PATTERNS; PERFORMANCE; PFGE; phage; phage type; phage types; Phenotype; pig; PIGS; PORK; POULTRY; Prevalence; prevention; PRINCIPLE; priority journal; PROFILE; PROFILES; PROTECTION; PROTOCOL; Public; Public Health; pulsed field gel electrophoresis; QUALITY; quantitative; RAW; reduction; reference; Reference Laboratory; REGION; regulation; Report; Research; resistance; RESTRICTION; retail; Risk; Risk Assessment; RISK-ASSESSMENT; Safety; SALMONELLA; Salmonella enterica; Salmonella enterica subsp.enterica serovar Infantis; Salmonella infantis; salmonellosis; SCIENCE; SEQUENCE; serotype; SEROTYPES; serovar; SEROVARS; slaughter; SLAUGHTER PIGS; species; standard; standardization; standards; STATE; STRAIN; STRAINS; studies; study; Suidae; SURVEILLANCE; survey; SUSCEPTIBILITY; Swine; SYSTEM; TARGET; TESTS; transmission; trends; Turkey; TYPHIMURIUM; typing; TYPING METHODS; values; veterinary; Veterinary Medicine; VETERINARY-MEDICINE; virulotyping; WHO; World Health; Zoonoses
DDC-Sachgruppe der DNB:: 610 Medizin, Gesundheit, Ernährung
Link URL:: http://online.liebertpub.com/doi/abs/10.1089/fpd.2011.1038
Einrichtung:: Bundesinstitut für Risikobewertung, Bundesinstitut für Risikobewertung (bis Juni 2014), Abteilung 4 - Biologische Sicherheit (bis Juni 2014), Fachgruppe 46 - Antibiotikaresistenz und Resistenzdeterminanten