Molecular identification of extended-spectrum-ß-lactamase genes from Enterobacteriaceae isolated from healthy human carriers in Switzerland

Geser,N.; Stephan,R.; Korczak,B.M.; Beutin,L.; Hächler,H.

doi:10.1128/AAC.05539-11

referiert

Veröffentlicht

Molecular identification of extended-spectrum-ß-lactamase genes from Enterobacteriaceae isolated from healthy human carriers in Switzerland

Geser,N.; Stephan,R.; Korczak,B.M.; Beutin,L.; Hächler,H.

In this study, fecal samples from 586 healthy humans were investigated to determine the occurrence of extended-spectrum-+¦-lactamase (ESBL)-producing Enterobacteriaceae in Swiss people. A total of 5.8% of the human fecal samples yielded ESBL producers, and all of the 34 isolated strains were Escherichia coli. PCR analysis revealed that 14 strains produced CTX-M-15, 10 produced CTX-M-1, 7 strains produced CTX-M-14, and 2 strains produced CTX-M-2 ESBLs. One strain produced SHV-12 ESBL. Of the 34 isolates, 15 produced additional TEM-1 broad-spectrum +¦-lactamases. By serotyping, a high degree of diversity among the strains was found. Copyright -® 2012, American Society for Microbiology. All Rights Reserved

Dateien

beschränkter Zugriff

Einordnung

Erschienen in:: Antimicrobial agents and chemotherapy
Vol. 56, H. 3 (2012), S. 1609-1612
Band:: 56
Heft:: 3
Datum der Veröffentlichung:: 2012
DOI:: 10.1128/AAC.05539-11
Sprache:: Englisch
Ressourcentyp:: Text
Schlagwörter:: 37; ACID; Adult; Africa; AGENT; AGENTS; amoxicillin plus clavulanic acid; ampicillin; analysis; animal; Animals; ANTIBIOTIC-RESISTANCE; antibiotic; antibiotic resistance; antibiotic sensitivity; antimicrobial susceptibility; AREA; article; assessment; Austria; bacterial strain; Berlin; BETA-LACTAMASE; beta-Lactamases; beta lactamase CTX M; beta lactamase CTX M 1; beta lactamase CTX M 14; beta lactamase CTX M 15; beta lactamase CTX M 2; beta lactamase SHV; beta lactamase SHV 12; beta lactamase TEM 1; beta lactamases; CAR; Carrier State; Cattle; cefalotin; cefepime; cefotaxime; cefoxitin; cefpodoxime; ceftazidime; cephalosporin; Cephalosporins; characterization; chicken; CHICKEN MEAT; Ciprofloxacin; class 1 integrons; clavulanic acid; clinical; COLI; COLONIZATION; COMMUNITY; comparison; cotrimoxazole; Denmark; DERIVATIVES; detection; DIFFERENCE; DISEASE; DISEASES; disk diffusion; DIVERSITY; DNA; DNA,Bacterial; DRUG; Drug Resistance; effect; EFFECTS; EMERGENCE; ENTEROBACTERIACEAE; ENVIRONMENT; Environmental; Environmental Microbiology; ENZYME; ENZYMES; epidemiology; ESBLs; ESCHERICHIA-COLI; Escherichia; Escherichia coli; Escherichia coli Infections; Evolution; extended spectrum beta lactamase; extended spectrum beta lactamase producing Enterobacteriaceae; FACILITIES; farm; fecal samples; FECES; feces analysis; Female; Food; FOOD ANIMALS; food safety; FOOD SAMPLES; Foodborne; France; gene; GENES; Genes encoding; Genetic Variation; genetics; Genotype; gentamicin; Germany; Groups; HEALTH; Health Policy; Human; Humans; hygiene; IDENTIFICATION; INFECTION; infectious disease; integron; Integrons; international; isolation; Japan; kanamycin; Klebsiella; Laboratories; Male; Meat; method; methods; Microbial Sensitivity Tests; microbiology; MICROORGANISMS; Middle Aged; MILK; minimum inhibitory concentration; molecular epidemiology; molecular genetics; multiplex-PCR; MULTIPLEX PCR; nonhuman; Norway; PATIENT; PCR; PERFORMANCE; PERSISTENCE; Phenotype; pig; PIGS; Plasmid; Plasmids; Polymerase Chain Reaction; polymyxin; POULTRY; Prevalence; priority journal; production; Prospective Studies; rapid detection; RAW; reference; Reference Laboratory; resistance; resistance genes; retail; RISK-ASSESSMENT; Risk; Risk Assessment; risk factor; Safety; SAMPLES; serotype; Serotyping; SEWAGE; slaughter; Spain; SPREAD; standard; standards; STRAIN; STRAINS; studies; study; survey; SUSCEPTIBILITY; Swine; Switzerland; Testing; Tetracycline; Travel; unclassified drug; veterinary; VOLUNTEERS
DDC-Sachgruppe der DNB:: 570 Biowissenschaften, Biologie
Link URL:: http://aac.asm.org/content/56/3/1609
Einrichtung:: Bundesinstitut für Risikobewertung, Bundesinstitut für Risikobewertung (bis Juni 2014), Abteilung 4 - Biologische Sicherheit (bis Juni 2014), Fachgruppe 41 - Mikrobielle Toxine