VP6-sequence-based cutoff values as a criterion for rotavirus species demarcation

Matthijnssens, J.; Otto, Peter H.; Ciarlet, M.; Desselberger, U.; van Ranst, M.; Johne, R.

doi:10.1007/s00705-012-1273-3

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VP6-sequence-based cutoff values as a criterion for rotavirus species demarcation

Matthijnssens, J.; Otto, Peter H.; Ciarlet, M.; Desselberger, U.; van Ranst, M.; Johne, R.

Indirect immunofluorescence techniques targeting the rotavirus (RV) protein VP6 are used to differentiate RV species. The ICTV recognizes RV species A to E and two tentative species, F and G. A potential new RV species, ADRV-N, has been described. Phylogenetic trees and pairwise identity frequency graphs were constructed with more than 400 available VP6 sequences and seven newly determined VP6 sequences of RVD strains. All RV species were separated into distinct phylogenetic clusters. An amino acid sequence cutoff value of 53% firmly permitted differentiation of RV species, and ADRV-N was tentatively assigned to a novel RV species H (RVH). -® 2012 Springer-Verlag

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Erschienen in:: Archives of Virology : Official Journal of the Virology Division of the International Union of Microbiological Societies
Vol. 157, H. 6 (2012), S. 1177-1182
Band:: 157
Heft:: 6
Datum der Veröffentlichung:: 2012
DOI:: 10.1007/s00705-012-1273-3
Sprache:: Englisch
Ressourcentyp:: Text
Schlagwörter:: ACID; Adult; Adult diarrheal rotavirus; amino acid; Amino Acid Sequence; AMPLIFICATION; analysis; animal; animal disease; Animals; ANTIGEN; ANTIGENS; Antigens,Viral; article; assessment; Bacterial Infections; Berlin; biology; bird disease; bovine; Capsid; capsid protein; Capsid Proteins; characterization; chemistry; chicken; Chickens; classification; clinical; cloning; CLUSTER; criteria; Determination; development; diagnostic; diagnostics; DIARRHEA; DIFFERENTIATION; Environmental Microbiology; epidemiology; evaluation; Evolution; FEATURES; Gastroenteritis; gene; genetics; genome; genomic; Germany; Groups; HAs; HEALTH; Human; IDENTITY; immunology; INFECTION; INFECTIONS; international; isolation and purification; Laboratories; lamb; method; METHODOLOGY; methods; microbiology; MOLECULAR CHARACTERIZATION; molecular genetics; Molecular Sequence Data; ORIGIN; OUTBREAK; paper; papillomaviruses; Phylogeny; Polyomaviridae; porcine; potential; Poultry Diseases; protein; Proteins; reassortment; RECOMMENDATIONS; reference; Report; Research; RESTRICTION; Risk; Risk Assessment; RISK-ASSESSMENT; rotavirus; Rotavirus infection; Rotavirus Infections; Rotaviruses; SEQUENCE; Sequence Homology; Sequence Homology,Amino Acid; SEQUENCE-ANALYSIS; SEQUENCES; Sequencing; SINGLE; species
DDC-Sachgruppe der DNB:: 610 Medizin, Gesundheit, Ernährung
Einrichtung:: Bundesinstitut für Risikobewertung, Bundesinstitut für Risikobewertung (bis Juni 2014), Abteilung 4 - Biologische Sicherheit (bis Juni 2014), Fachgruppe 42 - Lebensmittelhygiene und Sicherheitskonzepte
Einrichtung:: Friedrich-Loeffler-Institut, Institut für Bakterielle Infektionen und Zoonosen
Physischer Standort:: SD/2012/92