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Comparison of two PCR systems for the rapid detection of Leptospira spp. from kidney tissue

Mayer-Scholl, A.; Draeger, A.; Luge, E.; Ulrich, Rainer GND; Nöckler, K.

In this study we compared two routine PCR systems for the detection of Leptospira spp. and assessed their performance when directly applied to kidney samples from small mammals. Although the kappa value of 0.9 indicated a high level of agreement between the tests, the outer membrane lipoprotein gene lipl32 based PCR was more robust and showed a higher number of positive kidney samples

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Published in:
Current microbiology
Vol. 62, H. 4 (2011), p. 1104-1106
Date Issued:
2011
DOI:
10.1007/s00284-010-9829-5
Language:
English
Type of Resource:
Text
Keywords:
PCR assay; Leptospira
DDC subject of DNB:
610 Medical sciences, Medicine, Nutrition
Institution:
German Federal Institute for Risk Assessment, Predecessor Institution, Bundesinstitut für Risikobewertung (bis Juni 2014), Abteilung 4 - Biologische Sicherheit, Fachgruppe 45 - Diagnostik, Genetik und Erregercharakterisierung
Institution:
Friedrich-Loeffler-Institut, Institute of Novel and Emerging Infectious Diseases
Physical Location:
SD/2011/137

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Mayer-Scholl, A. / Draeger, A. / Luge, E. / et al: Comparison of two PCR systems for the rapid detection of Leptospira spp. from kidney tissue. 2011.

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