Rolling-circle amplification of viral DNA genomes using phi29 polymerase

Johne, R.; Muller, H.; Rector, A.; van Ranst, M.; Stevens, H.

doi:10.1016/j.tim.2009.02.004

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Rolling-circle amplification of viral DNA genomes using phi29 polymerase

Johne, R.; Muller, H.; Rector, A.; van Ranst, M.; Stevens, H.

Techniques for the single-step amplification of whole genomes have been developed into powerful tools for phylogenetic analyses, epidemiological studies and studies on genome organization. Recently, the bacteriophage phi29 DNA polymerase has been used for the efficient amplification of circular DNA viral genomes without the need of specific primers by a rolling-circle amplification (RCA) mechanism. Various protocols have been applied for detection of novel viruses, for differentiation between circular and linear forms of viral genomes and for generation of infectious genomic clones directly from specimens. Here, we summarize the broad application of the RCA technique to DNA viruses infecting humans, animals and plants

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Erschienen in:: Trends in microbiology : virulence, infection and pathogenesis
Vol. 17, H. 5 (2009), S. 205-211
Band:: 17
Heft:: 5
Datum der Veröffentlichung:: 2009
DOI:: 10.1016/j.tim.2009.02.004
Sprache:: Englisch
Ressourcentyp:: Text
Schlagwörter:: AMPLIFICATION; Analyse; animal; Animals; article; assessment; Bacteriophages; Berlin; detection; DIFFERENTIATION; DNA; DNA Replication; DNA Viruses; DNA,Circular; Dna,Viral; DNA-Directed DNA Polymerase; enzymology; FORM; genetics; genome; genomic; Germany; HAs; Human; Humans; isolation & purification; MECHANISM; metabolism; Models,Biological; ORGANIZATION; phi29 dna polymerase; PLANT; Plants; protein; Proteins; PROTOCOL; Research; review; Risk; Risk Assessment; RISK-ASSESSMENT; ROLLING-CIRCLE AMPLIFICATION; SPECIMENS; studies; study; technique; Viral Proteins; virology; VIRUSES
DDC-Sachgruppe der DNB:: 600 Technik
Einrichtung:: Bundesinstitut für Risikobewertung, Bundesinstitut für Risikobewertung (bis Juni 2014), Abteilung 4 - Biologische Sicherheit (bis Juni 2014), Fachgruppe 42 - Lebensmittelhygiene und Sicherheitskonzepte