Appraisal of the sensitising potential of orally and dermally administered mercaptobenzothiazol by a biphasic protocol of the local lymph node assay

Ahuja, V.; Wanner, R.; Platzek, T.; Stahlmann, R.

Mercaptobenzothiazole (MBT) is used while manufacturing natural rubber products. Our study deals with assessing its allergenic potential following dermal and oral routes of exposure, using a biphasic local lymph node assay (LLNA). Female Balb/c mice were treated with MBT (dermally 3, 10, 30% concentrations in DMSO; orally 1, 10, 100 mg/kg doses in corn oil) on the back (dermal study) or through oral administration (oral study) on days 1-3 followed by auricular application of 3, 10 and 30% concentrations, respectively, on days 15-17. End points determined on day 19 included ear thickness, ear punch weight, lymph node weight, lymph node cell count, and lymphocyte subpopulations (CD4+, CD8+, CD45+). After dermal application of 3% or 10% solution, a signiWcant increase in cell count and lymph node weight along with signiWcant decrease in CD8+ cells was observed. After initial oral administration of 1 mg/kg, we noticed a signiWcant ampliWcation in cell count. Following oral administration of 10 mg/kg, we observed a similar increase in cell count and lymph node weight. The results of our study show that the modiWed biphasic LLNA protocol can be used to study the sensitising potential of a compound also following the oralroute of exposure.

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Ahuja, V. / Wanner, R. / Platzek, T. / et al: Appraisal of the sensitising potential of orally and dermally administered mercaptobenzothiazol by a biphasic protocol of the local lymph node assay. 2009.

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