Genetic and functional properties of the self-transmissible Yersinia enterocolitica plasmid pYE854, which mobilizes the virulence plasmid pYV

Hammerl, J. A.; Klein, I.; Lanka, E.; Appel, B.; Hertwig, S.

doi:10.1128/JB.01467-07

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Genetic and functional properties of the self-transmissible Yersinia enterocolitica plasmid pYE854, which mobilizes the virulence plasmid pYV

Hammerl, J. A.; Klein, I.; Lanka, E.; Appel, B.; Hertwig, S.

Yersinia strains frequently harbor plasmids, of which the virulence plasmid pYV, indigenous in pathogenic strains, has been thoroughly characterized during the last decades. Yet, it has been unknown whether the nonconjugative pYV can be transferred by helper plasmids naturally occurring in this genus. We have isolated the conjugative plasmids pYE854 (95.5 kb) and pYE966 (70 kb) from a nonpathogenic and a pathogenic Yersinia enterocolitica strain, respectively, and demonstrate that both plasmids are able to mobilize pYV. The complete sequence of pYE854 has been determined. The transfer proteins and oriT of the plasmid reveal similarities to the F factor. However, the pYE854 replicon does not belong to the IncF group and is more closely related to a plasmid of gram-positive bacteria. Plasmid pYE966 is very similar to pYE854 but lacks two DNA regions of the larger plasmid that are dispensable for conjugation

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Erschienen in:: Journal of bacteriology
Vol. 190, H. 3 (2008), S. 991-1010
Band:: 190
Heft:: 3
Datum der Veröffentlichung:: 2008
DOI:: 10.1128/JB.01467-07
Sprache:: Englisch
Ressourcentyp:: Text
Schlagwörter:: Amino Acid Motifs; Amino Acid Sequence; Animals; article; Bacteria; Bacterial Proteins; Base Sequence; Berlin; Conjugation,Genetic; DNA; ENTEROCOLITICA; F Factor; Gene Transfer,Horizontal; genetics; Germany; HAs; Humans; metabolism; Molecular Sequence Data; Mutation; PATHOGENIC YERSINIA; pathogenicity; Plasmid; Plasmids; protein; Proteins; REGION; Replication Origin; Research; Risikobewertung; SEQUENCE; Sequence Analysis,DNA; STRAIN; STRAINS; VIRULENCE; VIRULENCE PLASMID; YERSINIA; Yersinia enterocolitica; YERSINIA-ENTEROCOLITICA
DDC-Sachgruppe der DNB:: 610 Medizin, Gesundheit, Ernährung
Link URL:: http://jb.asm.org/content/190/3/991
Einrichtung:: Bundesinstitut für Risikobewertung, Bundesinstitut für Risikobewertung (bis Juni 2014), Abteilung 4 - Biologische Sicherheit (bis Juni 2014)
Einrichtung:: Bundesinstitut für Risikobewertung, Bundesinstitut für Risikobewertung (bis Juni 2014), Abteilung 4 - Biologische Sicherheit (bis Juni 2014), Fachgruppe 45 - Diagnostik, Genetik und Erregercharakterisierung