Neuraminidase inhibitor susceptibility of porcine H3N2 influenza A viruses isolated in Germany between 1982 and 1999

Bauer, K.; Schrader, C.; Süss, Jochen; Wutzler, P.; Schmidtke, M.

doi:10.1016/j.antiviral.2007.03.007

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Neuraminidase inhibitor susceptibility of porcine H3N2 influenza A viruses isolated in Germany between 1982 and 1999

Bauer, K.; Schrader, C.; Süss, Jochen ; Wutzler, P.; Schmidtke, M.

As an intermediate host of avian and human influenza A viruses (FLUAV) pigs may play a potential role in interspecies virus transmission and reassortment of viral genes including those conferring antiviral drug resistance. Porcine FLUAV isolated in Germany between 1989 and 2001 contains mutations in the M2 gene inducing amantadine resistance. No data exist on neuraminidase inhibitor (NAI) susceptibility of these porcine FLUAV. We studied the antiviral activity of NAI against seven selected H3N2 FLUAV isolated from pigs in Germany between 1982 and 1999. All isolates were susceptible towards oseltamivir and zanamivir in neuraminidase enzyme-inhibition assays. Both compounds inhibited virus spreading and reduced the virus yields and plaque size at low concentrations. Higher concentrations were necessary to reduce the plaque number. Two isolates that differed in glycosylation pattern of viral hemagglutinin (HA) showed markedly reduced drug susceptibility in cell culture-based assays

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Erschienen in:: Antiviral research
Vol. 75, H. 3 (2007), S. 219-226
Band:: 75
Heft:: 3
Datum der Veröffentlichung:: 03.04.2007
DOI:: 10.1016/j.antiviral.2007.03.007
Sprache:: Englisch
Ressourcentyp:: Text
Schlagwörter:: A VIRUSES; AGENT; Amantadine; Amino Acid Substitution; Animals; antagonists & inhibitors; Antiviral Agents; article; ASSAY; Cell Line; chemistry; compounds; concentration; drug effects; Drug Resistance,Viral; Enzyme Inhibitors; gene; Germany; Hemagglutination,Viral; Hemagglutinin Glycoproteins,Influenza Virus; Human; Influenza; Influenza A Virus,H3N2 Subtype; isolation & purification; metabolism; Mutation; Neuraminidase; NUMBER; Oseltamivir; pharmacology; pig; porcine; potential; reassortment; Research; resistance; SUSCEPTIBILITY; Swine; THERAPY; transmission; virology; VIRUSES; Zanamivir
DDC-Sachgruppe der DNB:: 570 Biowissenschaften, Biologie
Link URL:: http://www.sciencedirect.com/science/article/pii/S0166354207002598
Einrichtung:: Bundesinstitut für Risikobewertung, Bundesinstitut für Risikobewertung (bis Juni 2014), Abteilung 4 - Biologische Sicherheit (bis Juni 2014), Fachgruppe 42 - Lebensmittelhygiene und Sicherheitskonzepte
Einrichtung:: Friedrich-Loeffler-Institut, Institut für Bakterielle Infektionen und Zoonosen
Physischer Standort:: SD/2007/45