Expression patterns and role of the CadF protein in Campylobacter jejuni and Campylobacter coli

Krause-Gruszczynska, M.; Alphen, L. B. van; Oyarzabal, O. A.; Alter, T.; Hänel, Ingrid; Schliephake, A.; Konig, W.; Putten, J. P. M. van; Konkel, M. E.; Backert, S.

doi:10.1111/j.1574-6968.2007.00802.x

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Expression patterns and role of the CadF protein in Campylobacter jejuni and Campylobacter coli

Krause-Gruszczynska, M.; Alphen, L. B. van; Oyarzabal, O. A.; Alter, T.; Hänel, Ingrid ; Schliephake, A.; Konig, W.; Putten, J. P. M. van; Konkel, M. E.; Backert, S.

Binding of Campylobacter jejuni and Campylobacter coli to host fibronectin is mediated by the 37 kDa outer membrane protein CadF. Immunoblot analysis of 58 C. jejuni and C. coli isolates of human and animal origin showed that CadF is expressed in every strain. In most C. jejuni isolates, a 37 kDa band (p37) and a less-prominent 32 kDa band (p32) reacted with the antibodies. In C. coli isolates, CadF was consistently larger with sizes of 39 kDa (p39) and 34 kDa (p34), respectively. PCR analysis and sequencing revealed the presence of a 39-bp insertion sequence in the cadF gene of C. coli strains, explaining the increased molecular size. Infection assays revealed that C. jejuni bound and invaded INT-407 epithelial cells much more efficiently than C. coli and that this difference was considerably reduced in isogenic cadF mutants. These results demonstrate that CadF is an important pathogenicity factor. The difference between CadF of C. jejuni and C. coli may potentially be exploited to discriminate these species in food and clinical specimens

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Erschienen in:: FEMS Microbiology Letters
Vol. 274, H. 1 (2007), S. 9-16
Band:: 274
Heft:: 1
Datum der Veröffentlichung:: 2007
DOI:: 10.1111/j.1574-6968.2007.00802.x
Sprache:: Englisch
Ressourcentyp:: Text
Schlagwörter:: 37; ADHESION; analysis; animal; Antibodie; article; Assay; assessment; Berlin; CadF; Campylobacter; Campylobacter coli; Campylobacter jejuni; cell; COLI; DIFFERENCE; Epithelial Cells; EXPRESSION; EXPRESSION PATTERNS; fibronectin; FIBRONECTIN-BINDING PROTEIN; FIELD GEL-ELECTROPHORESIS; Food; gene; Germany; Human; HUMAN INTESTINAL-CELLS; IDENTIFICATION; INFECTION; INT-407 CELLS; INTERNALIZATION; invasion; JEJUNI; MEMBRANE-PROTEIN; MOLECULAR-SIZE; MUTANTS; ORIGIN; PATHOGEN; PATHOGENESIS; pathogenicity; PATTERN; PCR; POULTRY; protein; PURIFICATION; reference; Risk; Risk Assessment; Safety; SEQUENCE; Sequencing; species; STRAIN; TIME; VIRULENCE
DDC-Sachgruppe der DNB:: 570 Biowissenschaften, Biologie
Link URL:: http://onlinelibrary.wiley.com/doi/10.1111/j.1574-6968.2007.00802.x/abstract?
Einrichtung:: Bundesinstitut für Risikobewertung, Bundesinstitut für Risikobewertung (bis Juni 2014), Abteilung 4 - Biologische Sicherheit (bis Juni 2014)
Einrichtung:: Friedrich-Loeffler-Institut, Institut für Bakterielle Infektionen und Zoonosen