Imprecise excision of insertion element IS5 from the fliC gene contributes to flagellar diversity in Escherichia coli

Strauch, E.; Beutin, L.

doi:10.1111/j.1574-6968.2006.00100.x

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Imprecise excision of insertion element IS5 from the fliC gene contributes to flagellar diversity in Escherichia coli

Motile strains of Escherichia coli K12 carrying both a chromosomal fliC-H48 gene and a plasmid encoded fliC-H4 gene express both types of flagellins, which are coassembled into functional flagella. By using flagellar-H48-specific antiserum and a plasmid curing procedure, nonmotile mutants were found that carried an IS5 insertion in the chromosomal fliC-H48 gene. Motile revertants were isolated that showed deletions of the IS5 element together with sections of the fliC-H48 gene resulting in an altered flagellar serotype in these strains. As IS5 elements were found associated with 35 of 53 known H-types in wildtype E. coli strains, this insertion element might play a major role in serotype diversity

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Erschienen in:: FEMS Microbiology Letters
Vol. 256, H. 2 (2006), S. 195-202
Band:: 256
Heft:: 2
Datum der Veröffentlichung:: 2006
DOI:: 10.1111/j.1574-6968.2006.00100.x
Sprache:: Englisch
Ressourcentyp:: Text
Schlagwörter:: ACTIVATION; Antibodies; article; assessment; Berlin; COLI; DIVERSITY; DNA transposable elements; Escherichia coli; Escherichia coli K12; ESCHERICHIA-COLI; EXCISION; Flagella; flagellin; gene; gene expression; gene rearrangement; Germany; INTEGRATION; MUTANTS; O-ANTIGEN; OPERON; protein; reference; Risk; Risk Assessment; RISK-ASSESSMENT; Safety; SEQUENCE; serotype; SEROTYPE O157-H7; Serotyping; STRAIN; STRAINS; TIME; TIMES
DDC-Sachgruppe der DNB:: 600 Technik
Link URL:: http://onlinelibrary.wiley.com/doi/10.1111/j.1574-6968.2006.00100.x/abstract
Einrichtung:: Bundesinstitut für Risikobewertung, Bundesinstitut für Risikobewertung (bis Juni 2014), Abteilung 4 - Biologische Sicherheit (bis Juni 2014)