Evaluation of a real-time PCR assay to detect Coxiella burnetii

Klee, S. R.; Ellerbrok, H.; Tyczka, J.; Franz, T.; Appel, B.

doi:10.1196/annals.1374.111

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Evaluation of a real-time PCR assay to detect Coxiella burnetii

Klee, S. R.; Ellerbrok, H.; Tyczka, J.; Franz, T.; Appel, B.

We evaluated real-time PCR assays for the detection of C. burnetii which targets sequences that are present either in one (icd) or in several copies (transposase of IS1111a) on the chromosome. The assays are highly sensitive, with reproducible detection limits of approximately 10 copies per reaction, at least 100 times more sensitive than capture ELISA, when performed on infected placenta material and specific for C. burnetii. The numbers of IS1111 elements in the genomes of 75 C. burnetii isolates were quantified by real-time PCR and proved to be highly variable

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Erschienen in:: Annals of the New York Academy of Sciences
Vol. 1078(2006), S. 563-565
Band:: 1078
Datum der Veröffentlichung:: 2006
DOI:: 10.1196/annals.1374.111
Sprache:: Englisch
Ressourcentyp:: Text
Schlagwörter:: article; ASSAY; Berlin; classification; Coxiella burnetii; DETECT; detection; diagnosis; ELISA; evaluation; genetics; genome; Germany; Humans; isolation & purification; NUMBER; NUMBERS; PCR; PCR assay; Polymerase Chain Reaction; Q Fever; REAL-TIME; real-time PCR; Safety; SEQUENCE; SEQUENCES; TARGET; TIME; TIMES; Transposases
DDC-Sachgruppe der DNB:: 610 Medizin, Gesundheit, Ernährung
Link URL:: http://onlinelibrary.wiley.com/doi/10.1196/annals.1374.111/abstract
Einrichtung:: Bundesinstitut für Risikobewertung, Bundesinstitut für Risikobewertung (bis Juni 2014), Abteilung 4 - Biologische Sicherheit (bis Juni 2014)